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André Boler Barros, PhD

@asbarros.bsky.social
316 followers 157 following 108 posts

Data-driven individual, trying to get by in the uncertainty of statistics and life. Avid Rstats follower. Data Analyst at GIMM Institute | PhD in Molecular Biosciences

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André Boler Barros, PhD @asbarros.bsky.social · 13/07/2026
"Our results suggest that Schrödinger’s causal inference” (33)—where studies avoid stating (or even explicitly deny) an interest in estimating causal effects yet are otherwise embedded with causal intent (...) is common in the observational health literature." pmc.ncbi.nlm.nih.gov/articles/PMC...
pmc.ncbi.nlm.nih.gov
Checking your browser - reCAPTCHA
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André Boler Barros, PhD @asbarros.bsky.social · 17/02/2026
scDesign3 - although this tool doesn't have a web interface, it explores additional scenarios, such as other data modalities or even multimodal experiments (github.com/SONGDONGYUAN...) #bioinformatics #scRNASeq
github.com
GitHub - SONGDONGYUAN1994/scDesign3: scDesign3 generates realistic in silico data for multimodal single-cell and spatial omics
scDesign3 generates realistic in silico data for multimodal single-cell and spatial omics - SONGDONGYUAN1994/scDesign3
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André Boler Barros, PhD @asbarros.bsky.social · 17/02/2026
SCOPIT - another tool with a web interface, it is focused on providing feedback on your experimental design but, now focusing on identification of cell types (alexdavisscs.shinyapps.io/scs_power_mu...)
alexdavisscs.shinyapps.io
SCOPIT, v1.1.4
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André Boler Barros, PhD @asbarros.bsky.social · 17/02/2026
scPower - Tool with a Web interface, that uses previous datasets to build a framework where you can vary the number of cells, samples - and even include budget value - to assess the impact on DEG results (scpower.helmholtz-muenchen.de/scpower/)
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André Boler Barros, PhD @asbarros.bsky.social · 17/02/2026
When searching for power analysis (or just want to check the ideal number of cells) for scRNASeq studies, here are some resources that may be relevant: #bioinformatics
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André Boler Barros, PhD @asbarros.bsky.social · 29/12/2025
"We need to create flexible, free paths to hiring (...). Imagine a model where capability, not how many hours students sit in class, is what matters; where demonstrated skills earn them credit and where employers recognize those credits as evidence of readiness to enter an apprenticeship program."
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André Boler Barros, PhD @asbarros.bsky.social · 29/12/2025
"[commit 1 percent of profits for workers re-training] isn’t charity. (...) Helping retrain workers is common sense, and such a small ask that these companies would barely feel it, while the public benefits could be enormous." www.nytimes.com/2025/12/27/o...
nytimes.com
Opinion | A 1 Percent Solution to the Looming A.I. Job Apocalypse
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André Boler Barros, PhD @asbarros.bsky.social · 26/11/2025
Simple yet very interesting challenge! Keep them coming #bioinfo #R #datascience
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André Boler Barros, PhD @asbarros.bsky.social · 26/11/2025
By selecting keywords that should be either in the gene set name or description, and using the R package 'msigdbr' (igordot.github.io/msigdbr/), voilá! github.com/andrebolerba... This folder contains a .qmd file and respective html report, with an excel file containing the final info dataset.
github.com
BitsandPieces/PathwayDatabase_Mining at main · andrebolerbarros/BitsandPieces
This repository stores codes & notebooks done in response to small & simple challenges - andrebolerbarros/BitsandPieces
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André Boler Barros, PhD @asbarros.bsky.social · 26/11/2025
The first challenge came up in a conversation about a project: how can I check gene sets across different databases (e.g., GO terms, KEGG Pathways, ...) involved in specific processes, like iron processing or lipid metabolism?
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André Boler Barros, PhD @asbarros.bsky.social · 26/11/2025
Today, I inaugurate a new Github repository: github.com/andrebolerba... I created this to store code that, although short and simple, may be useful for addressing specific needs and challenges.
github.com
GitHub - andrebolerbarros/BitsandPieces: This repository stores codes & notebooks done in response to small & simple challenges
This repository stores codes & notebooks done in response to small & simple challenges - andrebolerbarros/BitsandPieces
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André Boler Barros, PhD @asbarros.bsky.social · 19/11/2025
Congratulations Maria! Really interested to see you defend and to watch your future career in science!!
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André Boler Barros, PhD @asbarros.bsky.social · 18/11/2025
A special thank you to the PhD community, the facilities, and the community at IGC, now @gimmfoundation.bsky.social , for providing the right context for this thesis to see the light of day.
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André Boler Barros, PhD @asbarros.bsky.social · 18/11/2025
Thank you to everyone who supported me through this journey, from my supervisor @lferreiramoita.bsky.social , to my lab members - especially Henrique Colaço, @katiajesus.bsky.social and Elsa Seixas and, to the jury members - Vitor Cabral, Sarela García Santamarina, Nelson Frazao and Vera Martins.
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André Boler Barros, PhD @asbarros.bsky.social · 18/11/2025
I am very proud to announce that yesterday I have successfully defended my PhD. It was a long journey, filled with difficulties but with a dose of rewards - scientific, professional but most of all, personal. But, it definitely made me a better scientist, and a better person.
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André Boler Barros, PhD @asbarros.bsky.social · 28/10/2025
"(...) the rise of generative AI in bioinformatics has not diminished my role, but redefined it. It has challenged me to become a better scientist. For good or ill, AI seems to be here to stay. I urge you to embrace the technology — not to replace your expertise, but to amplify it. #bioinfo
nature.com
‘Am I redundant?’: how AI changed my career in bioinformatics
A run-in with some artefact-laden AI-generated analyses convinced Lei Zhu that machine learning wasn’t making his role irrelevant, but more important than ever.
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André Boler Barros, PhD @asbarros.bsky.social · 07/10/2025
Can't advise this lab more. If you'd like to work in a curious-driven and nurturing environment, with a high focus on robust data analysis, don't even think twice! #bioinfo #datascience
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André Boler Barros, PhD @asbarros.bsky.social · 30/09/2025
Last week, I was fortunate enough to watch a talk from @tkorem.bsky.social , where he presented different things, from addressing inter- study variability on microbiome projects to the use of novel approaches on metagenomics alignment and processing. Interesting and very relevant! #bioinfo
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André Boler Barros, PhD @asbarros.bsky.social · 29/09/2025
"GlucoStats demonstrates high efficiency in processing large-scale medical datasets in minimal time. Its modular design enables easy customization and extension, making it adaptable to diverse research and clinical needs" bmcbioinformatics.biomedcentral.com/articles/10.... #datascience #biostats
bmcbioinformatics.biomedcentral.com
Glucostats: an efficient Python library for glucose time series feature extraction and visual analysis - BMC Bioinformatics
Background The advancement of technology and continuous glucose monitoring (CGM) systems has introduced several computational and technical challenges for clinicians and researchers. The growing volume of CGM data necessitates the development of efficient computational tools capable of handling and processing this information effectively. This paper introduces GlucoStats, an open-source and multi-processing Python library designed for efficient computation and visualization of a comprehensive set of glucose metrics derived from CGM. It simplifies the traditionally time-consuming and error-prone process of manual CGM metrics calculation, making it a valuable tool for both clinical and research applications. Results Its modular design ensures easy integration into predefined workflows, while its user-friendly interface and extensive documentation make it accessible to a broad audience, including clinicians and researchers. GlucoStats offers several key features: (i) window-based time series analysis, enabling time series division into smaller ‘windows’ for detailed temporal analysis, particularly beneficial for CGM data; (ii) advanced visualization tools, providing intuitive, high-quality visualizations that facilitate pattern recognition, trend analysis, and anomaly detection in CGM data; (iii) parallelization, leveraging parallel computing to efficiently handle large CGM datasets by distributing computations across multiple processors; and (iv) scikit-learn compatibility, adhering to the standardized interface of scikit-learn to allow an easy integration into machine learning pipelines for end-to-end analysis. Conclusions GlucoStats demonstrates high efficiency in processing large-scale medical datasets in minimal time. Its modular design enables easy customization and extension, making it adaptable to diverse research and clinical needs. By offering precise CGM data analysis and user-friendly visualization tools, it serves both technical researchers and non-technical users, such as physicians and patients, with practical and research-driven applications.
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André Boler Barros, PhD @asbarros.bsky.social · 22/09/2025
"Delphi-2M predicts the rates of more than 1,000 diseases (...), with accuracy comparable to that of existing single-disease models. Delphi-2M (...) also enables sampling of synthetic future health trajectories" www.nature.com/articles/s41...
nature.com
Learning the natural history of human disease with generative transformers - Nature
Delphi-2M forecasts a person’s future health, covering more than 1,000 diseases, provides insights into co-morbidity dynamics and generates synthetic data for the training of AI models that have never...
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André Boler Barros, PhD @asbarros.bsky.social · 11/09/2025
Amazing repository with several references and resources for scRNASeq analysis github.com/crazyhottomm... #bioinfo #singlecell
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André Boler Barros, PhD @asbarros.bsky.social · 11/09/2025
"Even if work is done by or with the help of experts (...), it is crucial that researchers understand how a method works, that they can assess data quality, and that they fundamentally understand what types of conclusions can and cannot be drawn from their data" www.nature.com/articles/s41...
nature.com
Push-button science - Nature Methods
Technological advances change not only what we can learn as scientists, but also how science is conducted. Here we explore how automation and outsourcing are affecting the act of doing science.
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André Boler Barros, PhD @asbarros.bsky.social · 21/08/2025
Spreadsheets represent an everyday tool for most wet-lab scientists. So, why not use them at their highest potential, efficiently and ready for open science? This paper provides some recommendations for the use of spreadsheets: www.nature.com/articles/d41... #bioinfo #stats
nature.com
Six questions to ask before jumping into a spreadsheet
Spreadsheet software can be frustrating, but adopting some helpful habits can improve its effectiveness.
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Reposted by André Boler Barros, PhD
The Transmitter @thetransmitter.bsky.social · 13/08/2025
FlyBase, a Drosophila database, will lose a third of its team in early October because the Harvard grant that covered the employees’ salaries was canceled. Scientists warn that losing FlyBase could devastate fly research. By @claudia-lopez.bsky.social www.thetransmitter.org/community/ha...
thetransmitter.org
Harvard University lays off fly database team
The layoffs jeopardize this resource, which has served more than 4,000 labs for about three decades.
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André Boler Barros, PhD @asbarros.bsky.social · 12/08/2025
5/n The following paper provides an excellent overview of sequence-to-graph mapping algorithms—covering their principles, trade-offs, and performance benchmarks. It’s a great starting point if you’re interested on the topic genomebiology.biomedcentral.com/articles/10.... #bioinformatics #bioinfo
genomebiology.biomedcentral.com
A survey of sequence-to-graph mapping algorithms in the pangenome era - Genome Biology
A pangenome can reveal the genetic diversity across different individuals simultaneously. It offers a more comprehensive reference for genome analysis compared to a single linear genome that may intro...
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André Boler Barros, PhD @asbarros.bsky.social · 12/08/2025
4/n From equitable precision medicine to large-scale population studies and evolutionary research, pangenomics graphs and graph mapping excel in capturing diversity—especially in highly variable or repetitive genome regions where linear mapping fails.
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André Boler Barros, PhD @asbarros.bsky.social · 12/08/2025
3/n Unlike linear references, which favour the “reference” allele (known as reference bias), the integration of multiple haplotypes in pangenomic graphs and respective mapping reduces bias, improves alignment quality, and makes analyses fairer across diverse populations.
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André Boler Barros, PhD @asbarros.bsky.social · 12/08/2025
2/n Pangenome graphs represent variants as alternative paths, so reads can follow the path matching their true sequence. These graphs can represent SNPs, indels, structural variants, and complex rearrangements, all in one model—boosting variant calling accuracy for common and rare alleles.
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André Boler Barros, PhD @asbarros.bsky.social · 12/08/2025
1/n Because, in bioinformatics, sharing is caring, let me share something I have recently started exploring - graph mapping and pangenome graphs. A pangenome graph encodes a reference genome built from many genomes in one structure, thus trying to encapsulate the known genetic variability.
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
12/12 "It is an ethical imperative that research be designed and analysed to avoid wasting investment of animals, research dollars and effort". #biostatistics #stats
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
11/n Ultimately, understanding the C&N concepts, design experiments, and analytical approaches accounting for that are fundamental for rigorous and reproducible preclinical research.
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
10/n In a collaborative setting, another key element is a good and clear communication between the experimentalists and the data analysts. Only then can we have sound and robust experiments, with efficient analysis, that can really provide insight into the questions being asked.
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
9/n The analysis must reflect the data hierarchy (e.g., measurements nested within animals, animals within cages). Reporting statistical model details, including random effects and degrees of freedom adjustments, is also highly recommended.
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
8/n For the analysis, researchers can either aggregate data to a single summary per cluster (e.g., mean per cage) - which implies losing some information (the variation within a group can still be relevant) or use mixed or hierarchical models.
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
7/n Additionally, increasing the number of independent groups is generally more beneficial than having more animals per group.
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
6/n For experimental design, when a condition or treatment is applied, the concepts of blocking (e.g. ensuring that each cage have both treatments) and randomisation (e.g. in each cage, the animals that have each treatment is random) are essential.
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
5/n Researchers must account for these effects by considering the group (e.g. cage, litter, etc.) as the experimental unit for power calculations. Experimental design and downstream analyses should take into consideration this group effect as well.
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
4/n Repeated sampling data (repeated measures from the same individual) is also composed of nested observations - This is probably the scenario most easily recognised and accounted for in research.
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
3/n Examples of C&N include "cage effects" (e.g. mice housed in the same cage) , "litter effects" (animals from the same litter, thus sharing genetics or maternal environment), and "in vitro plate effects" (cells from the same plate).
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
2/n The interdependency of the obtained data points violates the assumptions of standard statistical tests like t-tests or ANOVA. Therefore, ignoring C&N can inflate Type I error rates, leading to false positives, thus undermining the validity of any conclusion obtained.
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André Boler Barros, PhD @asbarros.bsky.social · 26/06/2025
1/n Brief guide to statistical analysis of grouped data in preclinical research www.nature.com/articles/s42... In preclinical studies, clustering and nesting (C&N) scenarios, such as group-housed animals or cells on a single plate, are frequently found. This has important statistical implications
nature.com
A brief guide to statistical analysis of grouped data in preclinical research - Nature Metabolism
Clustering and nesting (C&N) arise in many preclinical studies, such as when animals are group-housed or share litters, or in cell culture. Ignoring C&N undermines the validity of analyses. He...
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Reposted by André Boler Barros, PhD
Wolfgang Huber @wkhuber.bsky.social · 24/06/2025
Instead of painstakingly dissecting a set of primary data to find novel patterns, it can be more effective to fit an unsupervised cluster-factor-latent-spaces model and then painstakingly dissect the model parameters to find patterns imposed by the model inference.
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André Boler Barros, PhD @asbarros.bsky.social · 17/06/2025
Very interesting paper from Soares lab @gimmfoundation.bsky.social
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André Boler Barros, PhD @asbarros.bsky.social · 28/05/2025
Reposting this a single time feels too short to show how much I agree with this
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André Boler Barros, PhD @asbarros.bsky.social · 27/05/2025
@symbiosisalumni.bsky.social
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André Boler Barros, PhD @asbarros.bsky.social · 27/05/2025
gimm.pt/jobs/researc... Great job opportunity for any bioinformatician out there! @gimmfoundation.bsky.social #bioinfo
gimm.pt
Research Assistant – GIMMResearch Assistant – GIMMsearch
Thank you very much for your generous donation and for sharing your testimony.We hope to more people feel inspired to join this mission of discovery and innovation!
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André Boler Barros, PhD @asbarros.bsky.social · 11/04/2025
This post was inspired by the amazing work of @sherlockpholmes.bsky.social and Paul J. McMurdie on rarefaction, as well as the study of rarefaction in alpha diversity by @amydwillis.bsky.social. #stats #bioinformatics
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André Boler Barros, PhD @asbarros.bsky.social · 11/04/2025
Some thoughts about rarefaction adaorg.github.io/datamisfits/...
adaorg.github.io
Rarefaction - Is it a good idea? – The Data Misfits
Rarefaction in microbiome studies - Is it a good idea or just a bad practice? What are the alternatives?
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André Boler Barros, PhD @asbarros.bsky.social · 11/04/2025
Interesting recommendations. However, it is still baffling to me the recommendation of rarefying samples to an even depth. Especially when so many statisticians have come out and demonstrated the problems it entails...
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André Boler Barros, PhD @asbarros.bsky.social · 09/04/2025
'One possible strategy is to organize consortium-led initiatives that integrate and curate reference datasets, establish criteria for dataset transparency, and mandate standardized preprocessing pipelines to reduce the variability in how competing models are evaluated' www.nature.com/articles/s41...
nature.com
A benchmarking crisis in biomedical machine learning - Nature Medicine
A lack of standardized benchmarks is hindering progress and patient benefits
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