iiinova — where your digital self keeps evolving

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 24/09/2026

#ThrowbackThursday with #AQLM! Registration for #AQLM2027 will open in the coming months. Stay tuned for application details and upcoming announcements. #ScopeTheScope 🔬 #MBL 🌊 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #STEMCommunity 🫂 #Scientist

052

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 15/09/2026

#AQLM Series 🧵: #Microscopy Mistakes -- Single Particle Tracking with Quantum Dots Single-particle tracking with quantum dots is highly sensitive to acquisition settings, labeling density, and localization precision. 🔬 📸 AQLM students learning SPT on a #Nikon AXR widefield system!

161

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 10/09/2026

The Loeb Laboratory at the #MBL has been central to #research and training since its construction in 1970. MBL courses held in Loeb have trained hundreds of scientists each year and continue to shape modern biological research. 📸 Original photo #AQLM 🧫 #Microscopy 🔬 #Imaging🪼#WoodsHole 🦞 #Optics 💡

010

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 27/08/2026

The Swope Center sits along Eel Pond, anchoring daily life at the #MBL. From meals and housing to informal discussions that extend beyond the lab, the Swope Center forms a space where science and community come together. #AQLM 🧫 #Microscopy 🔬 #WoodsHole 🦞 #STEMCommunity

010

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 13/08/2026

The Lillie Building at the #MBL was established in 1925 as a purpose-designed space for delicate scientific work. A century later, it remains a place where #microscopy, collaboration, and new ideas come together. 📸 Original photo #AQLM 🧫 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡

020

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 03/08/2026

#AQLM Series🧵: Microscopy Mistakes -- oh, FRAP! What happens after a #fluorescent molecule is bleached with a laser? In FRAP #microscopy, the recovery itself is the measurement. 🔬 📸 @3i Intelligent Imaging Innovations on the Marianas platform; 63× objective and a Photometrics Prime 95B camera. (1/7)

142

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 13/07/2026

Happy #MicroscopyMonday - #AQLM students doing single particle tracking using QD655 with THE Louise on a Leica DMi8 Inverted Widefield Microscope! Thank you for all your years at #AQLM, Louise! #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪

070

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 10/07/2026

Back by popular demand: Pets of AQLM, round two! Already looking ahead to the 2027 Pet Cohort. 🐾 #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥 #STEMCommunity 🫂

030

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 08/06/2026

From Eel Pond, a community of #microscopic life comes into view. Captured in darkfield at 4X, 0.16 NA on an #Evident DP75 microscope, each branching form is defined by the light it scatters, revealing delicate edges and structure against a dark field. #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬

150

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 05/06/2026

#ThrowbackThursday with #AQLM. Since its founding in 1980 by Shinya Inoué, AQLM has been closely tied to the evolution of #imaging living systems. Today, it continues to train new generations in quantitative biological imaging. #MBL 🌊 #Microscopy 🔬 #BioImaging 🧬 #WoodsHole 🦞 #Optics 💡#Confocal

091

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 10/05/2026

Happy #MothersDay from this shrimp and her eggs, captured on an #Evident DP75 4X/0.16 NA darkfield microscope. #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy

030

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 08/05/2026

That’s a wrap on #AQLM 2026 🔬Thank you to everyone who made this year’s course possible: students, faculty, TAs and vendors. We are looking forward to what this #microscopy community builds next. Interested in applying next year? 2027 applications open in late 2026, stay tuned for updates.

050

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 08/05/2026

It was an honor to hear Prof. Sarah Veatch, PhD, deliver yesterday’s Inoué Lecture, discussing how spatiotemporal pair-correlation analysis can recover nanoscale membrane organization in live-cell single-molecule localization #microscopy despite diffusion and molecular motion. #MBL 🌊 #AQLM 🧫

072

Reposted by Analytical and Quantitative Light Microscopy Course

Reto Fiolka @retof.bsky.social · 07/05/2026

Back from @aqlm.bsky.social . Adequate sunset view on the way back.

0121

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 07/05/2026

Why be out of focus when you could be at #AQLM, learning about microscopes inSTED? Today, our students did rotations with our vendors #OxfordInstruments, #Nikon, #3i, #Leica & #ONI, learning about super-resolution #imaging modalities including DNA-PAINT and dSTORM. #MBL 🌊 #Microscopy 🔬

070

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 06/05/2026

We tuned into a lecture by #AQLM course director, Prof. Diane Lidke, PhD, about single particle tracking. She discussed labelling strategies including quantum dots, and their applications using 2D/3D trajectory visualizations and diffusion mapping. #MBL 🌊 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼

0102

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 06/05/2026

Yesterday, we tuned into a lecture by Prof. Reto Fiolka, PhD, about Light-Sheet #Fluorescence #Microscopy. He discussed light sheet #optics and different #imaging modalities, as well as implementations in live imaging whole #embryos. #MBL 🌊 #AQLM 🧫 #BioImaging 🧬#WoodsHole 🦞 #LightMicroscopy 🧪

061

Reposted by Analytical and Quantitative Light Microscopy Course

Nat Prunet @nat-prunet.bsky.social · 05/05/2026

@carsten-wolff.bsky.social teaching @aqlm.bsky.social students about light sheet #microscopy on the @zeiss-microscopy.bsky.social LightSheet 7. Feat. cute crustacean embryo for specimen

0131

Reposted by Analytical and Quantitative Light Microscopy Course

Reto Fiolka @retof.bsky.social · 05/05/2026

@rtomer.bsky.social : Pizza time at AQLM! @aqlm.bsky.social !

263

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 05/05/2026

This sleek beam was created through a fluorescence cube on a Cairn OpenFrame, using a Toptica laser. ✨ #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥 #STEMCommunity 🫂

020

Reposted by Analytical and Quantitative Light Microscopy Course

Nat Prunet @nat-prunet.bsky.social · 05/05/2026

It’s #lightsheet #microscopy day @aqlm.bsky.social with @retof.bsky.social

1232

Reposted by Analytical and Quantitative Light Microscopy Course

Reto Fiolka @retof.bsky.social · 05/05/2026

@aqlm.bsky.social Quite reasonable requests…

0253

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 05/05/2026

Nickelback said to "look at this graph": A rail-mounted light sheet #microscope creating a sharp intensity peak. So cool! #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥

010

Reposted by Analytical and Quantitative Light Microscopy Course

3i @the.3i.social · 04/05/2026

We are @mblscience.bsky.social @aqlm.bsky.social sharing the latest live cell imaging technology with our Marianas SDC system. 3i's LaserStack, Vector3 TIRF/FRAP, and components from ...

155

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 04/05/2026

Real-time imaging at #AQLM, where “woooo got a signal” is a totally valid reaction! 🔬 #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥 #STEMCommunity 🫂 #ScientistLife 🧑‍🔬

060

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 03/05/2026

These bright “comets” mark growth, where EB3 tracking shows the advancing ends of microtubules in real time. ☄️ 📸 EB3 dynamics captured on the 3i Marianas #imaging system with a 63× objective and Prime BSI 95B camera. #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #Fluorescence🪼 #Optics 💡

050

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 03/05/2026

How your microscope spreads light: a guide to point spread functions. 💥 (1/5) 📸 #AQLM Day 4 Lab GIF! #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥 #STEMCommunity 🫂

130

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 02/05/2026

We attended “Digital Detectors” by Dr. Simon Watkins, PhD. Advances in sCMOS #technology reduce noise, improving sensitivity and dynamic range. With better coatings, Nyquist-matched pixels, and optimized illumination, light loss is minimized and image quality is improved. 🌊 #MBL #AQLM #Microscopy

010

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 02/05/2026

#Cells showing #mitochondria in red, actin in green, and nuclei in blue: a hustling, bustling city of powerhouses, scaffolds, and headquarters 🏭🛣🏢. Captured at 40× on an #Evident BX53 epifluorescence #microscope with a #Hamamatsu ORCA-Flash 4.0 LT3. #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Fluorescence🪼

030

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 02/05/2026

All planes in focus, all fields in view. 4×4 EDF mosaic from 16 z-stacks, captured with DIC at 20× on a #Nikon Eclipse NiE with a #Hamamatsu ORCA-Fusion camera. #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪

040

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 02/05/2026

Hydrozoa from Eel Pond @mblwoodshole imaged in dark field on an @evidentmicroscopy upright #microscope (cool to be able to tile and stitched on the go with a manual stage). These cuties are related to jellyfish, sea anemones and corals. 📸 Photo and caption credit: Nat Prunet @microscopic_farmer

0163

Reposted by Analytical and Quantitative Light Microscopy Course

Nat Prunet @nat-prunet.bsky.social · 01/05/2026

“We’re gonna need a bigger scope” Rabbit muscle fibers imaged with DIC @aqlm.bsky.social #microscopy #jaws @mblscience.bsky.social

0163

Reposted by Analytical and Quantitative Light Microscopy Course

Nat Prunet @nat-prunet.bsky.social · 01/05/2026

Polarization time! Rabbit muscle fibers imaged on a polarization microscope @aqlm.bsky.social #microscopy #labelfree

0122

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 01/05/2026

Pets of #AQLM 🐾 A look at the animals waiting for our students & faculty back home. #ScopeTheScope 🔬 #MBL 🌊 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️ #Confocal 🥼 #SuperResolution 🔥 #STEMCommunity 🫂 #ScientistLife 🧑‍🔬

020

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 01/05/2026

Two #photons are approaching a refraction grating. One photon says to the other: "Which slit should I go through?" The other says: "Don't be so particular." #ScopeTheScope 🔬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #FluorescenceMicroscopy ⭐️

040

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 01/05/2026

Moments from last night's #AQLM Lightning Talks ⚡️ This year’s cohort spans #PhD students, postdocs, #microscopy core directors, and professors, each bringing a mix of technical depth and perspective to every talk. #ScopeTheScope 🔬 #MBL 🌊 #Microscopy 🔬 #Optics 💡 #LightMicroscopy

030

Reposted by Analytical and Quantitative Light Microscopy Course

Nat Prunet @nat-prunet.bsky.social · 01/05/2026

Diverging light coming out of the condenser after illuminating the specimen #microscopy @aqlm.bsky.social

0141

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 01/05/2026

What makes #AQLM stand out is what builds in a few short days: by Day 3, people are no longer just classmates, but collaborators in the making. Early mornings at the beach and friendly discussions lay the groundwork for professional collaborations that extend well beyond the course. 🔬 #MBL 🦞

010

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 30/04/2026

#ScopeTheScope at #AQLM Day 2 -- we worked through data extracted from images using FIJI, with the lecture given by none other than fellow current AQLM student, Noelle Cataldo. #MBL 🌊 #Microscopy 🔬 #BioImaging 🧬#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #Confocal 🥼 #STEMCommunity 🫂

030

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 30/04/2026

After lunch, we tuned into a lecture by Dr. Stephen Ross, PhD. He emphasized that optimal resolution depends on precise objective manufacturing: proper alignment, high-quality coatings, and refractive index matching to the sample. These minimize distortion and optical aberrations in #microscopy.

190

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 30/04/2026

Good morning AQLMers, #ScopeTheScope at Day 2 of AQLM 🌊 Today, we observe wave optics in theory and wave behavior in practice, visible everywhere in Woods Hole. #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪#SuperResolution 🔥 #STEMCommunity 🫂

031

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 30/04/2026

It's important to find happiness in the *little* things in life. That's why we built a microscope from first principles today at the #AQLM course at the #MBL. Up next tomorrow are wave optics and image analysis 🔬 @mblscience.bsky.social #ScopeTheScope 🔬 #Microscopy 🧬 #WoodsHole 🦞 #Optics 💡

041

Reposted by Analytical and Quantitative Light Microscopy Course

Marine Biological Laboratory @mblscience.bsky.social · 29/04/2026

Just in time for warmer weather in Woods Hole, our first two cohorts of ARTC students have arrived to campus this week. Joining the FIR cohort, who arrived earlier this week, our AQLM students are starting to roll in. Welcome to the MBL!

073

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 29/04/2026

#ScopeTheScope at AQLM Day 1 : Ray optics with Prof. Alexa Mattheyses, PhD. Up next: Building a microscope in the very first lab 👀 @mattheyseslab.bsky.social #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #BioImaging 🧬 #Fluorescence🪼#WoodsHole 🦞 #Optics 💡 #LightMicroscopy 🧪 #STEMCommunity 🫂 #ScientistLife 🧑‍🔬

042

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 29/04/2026

Good morning, AQLMers! Today is the day - make sure you check in at Swope Center and pick up your badge before the Welcome Lecture. Drop a microscope in the comments if you’re already here! 🔬🔥 🛬 #MBL 🌊 #AQLM 🧫 #Microscopy 🔬 #WoodsHole 🦞 #Optics 💡

Modern concrete building at the Marine Biological Laboratory under a clear blue sky, with a blue sign in the foreground reading “Swope Center” beside a grassy lawn.

031

Reposted by Analytical and Quantitative Light Microscopy Course

Alexa Mattheyses @mattheyseslab.bsky.social · 20/02/2026

There are an extremely limited number of open slots for the Analytical and Quantitative Light Microscopy @aqlm.bsky.social course at @mblscience.bsky.social April 29-May 8 2026. www.mbl.edu/education/ad... Apply here for this amazing opportunity!

Microscopy image of keratin (cyan) and desmosomes (magenta)

099

Analytical and Quantitative Light Microscopy Course @aqlm.bsky.social · 30/01/2026

⏱️ Deadline to apply for AQLM 2026 is today! ⏱️ Get your applications in to be considered for this year's course. Make sure your recommenders submit their letters as well! #microscopy #imaging #fluorescence #biology #optics #AQLM

022