iiinova — where your digital self keeps evolving

Tony Mustoe @amustoe.bsky.social · 27/08/2026

Very cool study from @rdaslab.bsky.social in @science.org demonstrating the ability of AIs to design novel RNA psuedoknots www.science.org/doi/10.1126/.... Was honored to be asked to write a commentary on it and the broader state of RNA structure prediction www.science.org/eprint/KFKMB...

science.org

The knotty problem of RNA structure prediction

Artificial intelligence enables the design of RNA pseudoknots

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Reposted by Tony Mustoe

Das Lab @rdaslab.bsky.social · 27/08/2026

Our paper with @eternagame, 5 AI teams, and cryo-EM collabs on de novo design of #RNA pseudoknots with #deeplearning is out in @science.org! Comes with a provocative commentary on RNA structure prediction by @amustoe.bsky.social lab! doi.org/10.1126/scie... doi.org/10.1126/scie...

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Tony Mustoe @amustoe.bsky.social · 04/08/2026

It was such a pleasure writing this with @incarnatolab.bsky.social! There has been so much exciting progress in probing the complexity of RNA folding in cells. Although we couldn't cover it all, we did our best to highlight several major emerging areas. I’m excited to see where the field goes next!

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Tony Mustoe @amustoe.bsky.social · 21/04/2026

Thanks and congrats to lead authors Irfana Saleem and Thomas Miller and the other co-authors for their incredible work on this project.

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Tony Mustoe @amustoe.bsky.social · 21/04/2026

We are very excited about the long-term potential of msDMS-MaP to improve understanding of RNA biology and enable applications such as RNA targeted drug discovery. The experiments are remarkably easy, so if you are using DMS, I encourage you try out msDMS-MaP yourself!

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Tony Mustoe @amustoe.bsky.social · 21/04/2026

This additional data enables direct discovery and annotation of tertiary motifs and protein binding sites. In two examples in our paper, we use msDMS-MaP uncover new relationships between rRNA tertiary folding and ribosome assembly, and new protein binding sites on the human 7SK RNA in cells.

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Tony Mustoe @amustoe.bsky.social · 21/04/2026

msDMS-MaP is a simple extension of standard DMS probing experiments that helps solve this limitation. We developed a new strategy that can simultaneously measure multiple types of DMS modifications that report on RNA secondary structure (N1/N3) AND higher-order structure (N7-G).

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Tony Mustoe @amustoe.bsky.social · 21/04/2026

As many know, DMS probing experiments are amazing at mapping RNA secondary structure, but are less capable at identifying higher-order tertiary structures and protein binding sites. Because higher-order structures are often the most functionally interesting, this represents a significant limitation.

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Reposted by Tony Mustoe

From the Labs at Baylor College of Medicine @bcmfromthelabs.bsky.social · 21/04/2026

Check out the new method that reveals structures of #RNA-protein complexes in living cells. @amustoe.bsky.social @bcmhouston.bsky.social @cp-molcell.bsky.social www.eurekalert.org/news-release...

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