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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 17/03/2026

We "cirurgically" dissected the locus to demostrante that most of the effect was specifically due to the rpoB and rpoC genes within the operon! We uncoupled the dosage of rpoB and rpoC from the rest of the locus!!

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 17/03/2026

But apparently there are no major alterations in RNAP celular location upon rpoBC relocation! (a story to follow up using supreresolution!)

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 17/03/2026

that was accompanied by a reduction in mRNA and protein levels (and rifampocin sensitivity!)

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 17/03/2026

Relocation was accompanied by a reduction in gene dosage due to multi-fork replication!

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 17/03/2026

We relocated the whole locus to the chrosome termini in V. cholerae! (This bug has two chromosomes). We noticed a nutrient dependent reduction in fitness and growth rate upon rpoBC locus relocation!

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 17/03/2026

In fast growing bacteria, transcription and translation genes are in close proximity of oriC. The core of the sole RNA polymerase is encoded by rpoB and rpoC genes. They form a locus well conserved in the tree of life along with ribosomal proteins and tRNAS! What happens if we alter this?

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 25/02/2026

Celebrating Next paper by Larotonda et al. ! Really proud of our next work on positional Genetics of RNA polymerase. More news soon on this!!

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 08/01/2026

After inducing rearrangements and selecting for fast growth, we found dramatically reconfigured genomes with stable physiology despite altered gene order. This technique enabled on‑demand, large‑scale genome rearrangements—inversions, duplications, and translocations across megabases.

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 08/01/2026

We placed 14 loxPsym sites to enable inversions, duplications, and translocations while preserving near‑wild‑type fitness before rearrangement. Including essential genes allowed avoiding deletions.

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 29/05/2025

Budget cuts do not curb our enthusiasm! Ian lab meeting today on Bradyrhizobium cell physiology! #LabMeetinDay

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 26/05/2025

Escribime por DM. Si querés ver lo que hacemos visítanos en: asoler1.wixsite.com/iib-geb

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 26/05/2025

Estas en 3-4° año de biotecnología o carreras afines? Estamos buscando un/a estudiante de @unsamoficial.bsky.social para incorporar al laboratorio y presentar a una beca de entrenamiento de la CIC PBA! Te interesa? Vení a trabajar con nosotros!! #UNSAM #Biotecnologia !!

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 15/04/2025

The rpoB and rpoC genes are part of a large locus, and being the last ORF of the operon. To show that the phenotypes observed were consequence exclusively of RNA polymerase genes genomic location, we disected the locus.

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 15/04/2025

We then added a second copy of the locus to show that the pheno typic effects are mostly due to alteration in replication associated rpoBC dosage reduction.

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 15/04/2025

We built an rpoC-mCherry translational fusion that allowed us to observe that the distribution is not disturbed upon relocation. However, we notice less RpoC-mCherry protein when the locus is far from oriC.

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 15/04/2025

When rpoBC is far from oriC, growth rate and dosage decreases.

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 15/04/2025

Bacteria harbour a single RNAP encoded by rpoBC genes. In fast-growers it is conserver close to oriC (bsky.app/profile/did:...). We relocated rpoBC (in red) to different locations within Vibrio cholerae genome:

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 20/03/2025

Gran Curso de Biología Vegetal en Chile! Coordina el gran @estevezjosem.bsky.social

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Alf Soler-Bistué 🦠🔬🧫 @alfmicrobe.bsky.social · 17/12/2024

A new Argentinean Society for General Microbiology (SAMiGe) Board starts. My new role is General secretary. We will do our best effort to promote fundamental microbiology in 🇦🇷 and the region in this challenging context!

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