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Adam Glaser

@adamkglaser.bsky.social
1K followers 213 following 25 posts

Developing hardware + software tools for large-scale imaging at the Allen Institute for Neural Dynamics

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Adam Glaser @adamkglaser.bsky.social · 16/09/2026
Congrats to the multi-disciplinary Neural Dynamics neuromodulation team on Topographic structure and function of locus coeruleus noradrenaline neurons. www.nature.com/articles/s41...
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Adam Glaser @adamkglaser.bsky.social · 16/09/2026
Congrats to the Neural Dynamics team members on developing 3D-MAESTRO: A scalable, modular, portable pipeline for automated processing of large-scale volumetric brain microscopy data. www.biorxiv.org/content/10.6...
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Reposted by Adam Glaser
voigtvision.bsky.social @voigtvision.bsky.social · 20/08/2026
🤩 It's time for some invertebrate eye candy! 🤩 Here's a ctenophore (or comb jelly) stained with phalloidin by John Alexander Salazar Hernandez & imaged on an exaSPIM built by Kaelin Wulf of the team of @adamkglaser.bsky.social as part of the FHL Functional Biodiversity course #microscopy
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Adam Glaser @adamkglaser.bsky.social · 31/10/2025
For really big ExM tissues we made a teflon "drumhead" for one of our open-top systems that was inspired by the FEP flexvat bases for 3D printers. At high mag vibration could definitely be an issue though...
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Adam Glaser @adamkglaser.bsky.social · 01/10/2025
New 105 MP Sony IMX927 sensor (global shutter) capable of streaming data at 100 fps... 10.5 gigavoxels/sec. www.sony-semicon.com/en/products/...
sony-semicon.com
High Resolution, High-speed, 105 MP & 100 fps Global Shutter Image Sensor | Products & Solutions | Sony Semiconductor Solutions Group
Sony Semiconductor Solutions Group develops device business which includes Micro display, LSIs, and Semiconductor Laser, in focusing on Image Sensor.
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Adam Glaser @adamkglaser.bsky.social · 29/09/2025
Latest imaging data from our completely re-designed ExA-SPIM system. Expansion-assisted imaging (3X expansion, 0.3 NA) enables brain-wide imaging with high-contrast and <400 nm effective resolution.
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Adam Glaser @adamkglaser.bsky.social · 29/09/2025
That is a good data point to know. We hypothesize (no data yet) that aberrations will reduce quickly with expansion factor (RI gradients proportional to density, density gradients will reduce with expansion factor ^3). Whereas the imaging path length only increases linearly with expansion factor.
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Adam Glaser @adamkglaser.bsky.social · 29/09/2025
ExA-SPIM2 will provide 35 mm WD with 0.5 NA. I think there are definitely applications where higher NA is needed. If we designed a 3rd variant, I think aiming for ~0.8 - 0.85 NA (which allows for symmetric 90 deg objectives) with 20 mm WD would be very valuable.
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Adam Glaser @adamkglaser.bsky.social · 29/09/2025
"Expansion-assisted" is all about balancing light collection (NA^2) with practicality of engineering lenses with both high NA and long WD. We are collecting data now to quantify this, but we also believe aberrations in ExM will scale with NA^3. So IMO go as low in NA as specimen brightness allows!
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Adam Glaser @adamkglaser.bsky.social · 07/07/2025
First 3200 MP images from the LSST at the Vera C. Rubin Observatory! www.nature.com/articles/d41...
nature.com
First images from world’s largest digital camera leave astronomers in awe
The Rubin Observatory in Chile will map the entire southern sky every three to four nights.
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Adam Glaser @adamkglaser.bsky.social · 06/06/2025
High-throughput line scanning confocal microscopy www.biorxiv.org/content/10.1...
biorxiv.org
A parallelly distributed microscope and software system for scalable high-throughput multispectral 3D imaging
Recent advances in high throughput optical microscopy have achieved whole-organism scale imaging at diffraction-limited resolutions. Current microscopes, however, require making compromises between ac...
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Adam Glaser @adamkglaser.bsky.social · 20/05/2025
None of the above captures the long WD (35 mm). Perhaps we should introduce a volumetric SBP... that divides the usable WD by either the Rayleigh axial resolution, or an isotropic resolution assumption that divides by the lateral resolution -> would be very high for this lens.
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Adam Glaser @adamkglaser.bsky.social · 20/05/2025
Thanks for resolving the numbers. Agreed, this was the primary motivation for ExA-SPIM in the first place. More pixel parallelization with a larger SBP, either in 2D (global shutter) or even along 1D for rolling shutter (i.e. for ASLM). Also removes some scanning bottlenecks with a low FPS sensor.
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Adam Glaser @adamkglaser.bsky.social · 19/05/2025
That is right, I used the equation from doi: 10.1117/1.ap.3.4.044001. The required number of samples to fully resolve the signal - also their equation uses 0.5 instead of 0.61. Lens has more information than any current standard camera sensor but potentially could be fully used in the near future.
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Adam Glaser @adamkglaser.bsky.social · 19/05/2025
Totally agree with you 😆 space-bandwidth product > etendue (!) For 420 - 850 nm light the SBP is 175 - 825 megapixels. Around 500 megapixels at 500 nm.
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Adam Glaser @adamkglaser.bsky.social · 19/05/2025
We are excited to share our development of a new 10X lens with NA=0.50 over a 7.2 mm FOV and a 35 mm working distance (water), fully corrected from 400-850 nm. This lens will power our upcoming ExA-SPIM "2" and is available for dissemination - please get in touch if interested!
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Reposted by Adam Glaser
voigtvision.bsky.social @voigtvision.bsky.social · 16/05/2025
Looking forward to the @mesospim.bsky.social #lightsheet #Microscopy anniversary symposium in October with @preibischs.bsky.social @adamkglaser.bsky.social @christellelangevin.bsky.social & many more! And to the next mesoSPIM decade! 🔬 Thanks @nvladimus.bsky.social & Co for organizing it!
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Adam Glaser @adamkglaser.bsky.social · 02/05/2025
To look into: a Python alternative to ZEMAX... github.com/HarrisonKram...
github.com
GitHub - HarrisonKramer/optiland: Comprehensive optical design, optimization, and analysis in Python, including GPU-accelerated and differentiable ray tracing via PyTorch.
Comprehensive optical design, optimization, and analysis in Python, including GPU-accelerated and differentiable ray tracing via PyTorch. - HarrisonKramer/optiland
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Adam Glaser @adamkglaser.bsky.social · 17/04/2025
New review out on imaging 3D cell cultures. Congratulations to Huai-Ching and @jonliu123 on leading this excellent new review! www.nature.com/articles/s41...
nature.com
Imaging 3D cell cultures with optical microscopy - Nature Methods
This Review discusses current 2D and 3D microscopy methods for imaging three-dimensional cell cultures and emerging strategies to address key challenges.
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Adam Glaser @adamkglaser.bsky.social · 12/04/2025
Curved light-sheet microscopy www.nature.com/articles/s41...
nature.com
Curved light sheet microscopy for centimetre-scale cleared tissue imaging - Nature Photonics
Light sheet microscopy with curved light sheets enables tiling-free imaging of an entire intact cleared mouse brain with lateral and axial spatial resolutions of 1.0 μm and 2.5 μm, respectively, in le...
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Adam Glaser @adamkglaser.bsky.social · 11/04/2025
Thermal Lensing Effects in Light-Sheet Microscopy arxiv.org/abs/2504.02969
arxiv.org
Thermal Lensing Effects in Two-Photon Light-Sheet Microscopy
In light-sheet fluorescence microscopy (LSFM), the axial resolution is governed by the illumination beam profile, motivating the development of advanced beam-shaping techniques to enhance imaging perf...
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Adam Glaser @adamkglaser.bsky.social · 04/04/2025
Sound-sheet microscopy - in this study, we introduce nonlinear sound-sheet microscopy (NSSM), a method to image targeted biological functions across cubic centimeters of opaque living tissue. www.science.org/doi/10.1126/...
science.org
Nonlinear sound-sheet microscopy: Imaging opaque organs at the capillary and cellular scale
Light-sheet fluorescence microscopy has revolutionized biology by visualizing dynamic cellular processes in three dimensions. However, light scattering in thick tissue and photobleaching of fluorescen...
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Adam Glaser @adamkglaser.bsky.social · 05/03/2025
New SPIM system underway!
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Adam Glaser @adamkglaser.bsky.social · 21/02/2025
Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue www.biorxiv.org/content/10.1...
biorxiv.org
Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue
Light sheet microscopy is the ideal technique for multiscale imaging of large and cleared tissues, and it is desirable to achieve the highest possible isotropic resolution across the entire sample. Ho...
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Adam Glaser @adamkglaser.bsky.social · 28/01/2025
It’s across the board. All grants from all agencies, future ones on pause and current ones also on pause :/
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Adam Glaser @adamkglaser.bsky.social · 28/01/2025
Can maybe estimate based on the 35 mm full frame sensor size (36x24 mm). So probably around 1.46 micron pixels? The max rate is 3280 megapixels/sec 🤯
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Adam Glaser @adamkglaser.bsky.social · 28/01/2025
New 410 MP camera sensor coming in 2025 from Canon global.canon/en/news/2025...
global.canon
Canon develops CMOS sensor with 410 megapixels, the largest number of pixels ever achieved in a 35 mm full-frame sensor | Canon Global
TOKYO, January 22, 2025—Canon Inc. announced today that it has developed a CMOS sensor with 410 megapixels, which is the largest number1 of pixels ever achieved in a 35 mm full-frame sensor.
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