FRAP-in-SR for dynamic studies at 60nm resolution!! If you explore cell cycle, DNA damage or 53BP1, here are new tools for live-cell microscopy: a fruitful collaboration with @carlzeissstiftung.bsky.social for SIM2/diSIM and @rappopto.bsky.social for localised FRAP www.biorxiv.org/content/10.1...
biorxiv.org
FRAP-in-SR: Fluorescence recovery in the Super-Resolution regime reveals subcompartments of 53BP1 foci
We combine Lattice Structured Illumination Microscopy (diSIM/SIM2 with ~60 nm resolution), Lattice Light-sheet microscopy and Fluorescence Recovery After Photobleaching (FRAP) to explore 53BP1 dynamic...