Nejc Haberman @nejchaberman.bsky.social · 15/11/20246/10 Our analysis shows that AGO2 binds upstream of 3′UTR-derived RNA start sites—independent of miRNA binding. Interestingly, G4 motifs influence the binding positions of both AGO2 and UPF1, shaping the capping sites. 100 0
Nejc Haberman @nejchaberman.bsky.social · 15/11/20247/10 We also found that cytoplasmic capping can occur subsequent to AGO2/siRNA-mediated cleavage, by analysing FANTOM5 CAGE from siRNA-treated samples. Our findings demonstrate that capping can readily follow cytoplasmic cleavage, and is not exclusive to transcriptional initiation. 111 0
Nejc Haberman @nejchaberman.bsky.social · 15/11/20248/10 Using high-resolution imaging, we validated that 3′UTR-derived RNAs localise differently from their parental mRNAs within the cell, hinting at potential independent functions. 100 0
