Nejc Haberman @nejchaberman.bsky.social · 15/11/20245/10 By integrating AGO2-eiCLIP, eCLIP, and CAGE-seq, we found their origins linked to RNA-binding proteins, specifically AGO2 and UPF1. These sites are often rich in G-motifs capable of forming G-Quadruplexes. 111
Nejc Haberman @nejchaberman.bsky.social · 15/11/20246/10 Our analysis shows that AGO2 binds upstream of 3′UTR-derived RNA start sites—independent of miRNA binding. Interestingly, G4 motifs influence the binding positions of both AGO2 and UPF1, shaping the capping sites. 100
Nejc Haberman @nejchaberman.bsky.social · 15/11/20247/10 We also found that cytoplasmic capping can occur subsequent to AGO2/siRNA-mediated cleavage, by analysing FANTOM5 CAGE from siRNA-treated samples. Our findings demonstrate that capping can readily follow cytoplasmic cleavage, and is not exclusive to transcriptional initiation. 111
