Wildtype One @wildtypeone.bsky.social · 18/01/2026Researchers, Most irreproducible results don’t fail loudly They quietly rot (a thread) 110
Wildtype One @wildtypeone.bsky.social · 18/01/2026What almost no one checks first: • Passage number drift • FBS lot changes • Cell stress history (freeze–thaw, confluency, antibiotics) Same cell line name does NOT mean the same cell state (2/n) 110
Wildtype One @wildtypeone.bsky.social · 18/01/2026Rule of thumb: If your result disappears after thawing a new vial, You didn’t discover biology You discovered cell line drift Document passage Freeze early Repeat across thaws — Wildtype One 🧬 (3/n) 000
