iiinova — where your digital self keeps evolving

Replies

Gabriel Rocklin @grocklin.bsky.social · 19/11/2025

This work was led by the incredible Cydney Martell, who worked out the entire approach from scratch and did virtually all the experiments and computational analysis (and earned her own PhRMA fellowship)! phrmafoundation.org/grants-fello... With proteomics help from the Savas Lab at NU!

phrmafoundation.org

Cydney M. Martell

120

Gabriel Rocklin @grocklin.bsky.social · 19/11/2025

We expressed the protein domains together as a mixture in one E. coli culture, purified our mixture of domains and concentrated to 10mg/mL, then used TMT proteomics to monitor the change in soluble protein abundance after high temp/low pH.

120

Gabriel Rocklin @grocklin.bsky.social · 19/11/2025

Overall, 25-50% of the total protein became insoluble, but the proteomics revealed which proteins aggregated (became insoluble) more and which aggregated less.

110