iiinova — where your digital self keeps evolving

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Matt Lycas @lycasworks.bsky.social · 20/01/2026

🔬🚨New preprint alert! 🚨🔬 We developed quantitative expansion microscopy (qExM) - a method to accurately count proteins in situ by combining expansion microscopy's improved labeling with statistical estimators borrowed from ecology www.biorxiv.org/content/10.6... #SuperResolution #CellBiology

biorxiv.org

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Matt Lycas @lycasworks.bsky.social · 20/01/2026

How does qExM work? We use cryo-fixation + GMA anchoring to boost antibody labeling efficiency, then apply capture-recapture statistics to dual-labeled samples. The expansion makes epitopes more accessible while reducing label size relative to targets - perfect for quantitative imaging!

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Matt Lycas @lycasworks.bsky.social · 20/01/2026

We then used qExM to measure the abundance of mitochondrial respiratory chain complexes in situ! We measured the abundances for Complex I, III, and IV. How they differ in absolute abundances in metabolically distinct subpopulations And even how T-cell activation changes supercomplex density

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