Manuel Irimia @mirimiam.bsky.social · 30/12/2024To tackle this major open question, common to other tissue-specific splicing regulators, we generated CRISPR-Cas9 deletion mutants in zebrafish for 18 conserved neural-specific microexons regulated by srrm3/4, as well for srrm3 and srrm4. 4/9 100 0
Manuel Irimia @mirimiam.bsky.social · 30/12/2024We phenotypically characterized these microexon KO lines, along with those for srrm3 and srrm4, across multiple organization levels, including axon guidance, neurite growth, larval and juvenile locomotion, larval sensory responses, and social behavior in juveniles. 5/9 100 0
Manuel Irimia @mirimiam.bsky.social · 30/12/2024In contrast to the strong phenotypes observed for their master regulator srrm3, most microexon mutants showed no phenotypes for most tests, with a few notable exceptions. For instance, vti1 and kif1b KOs showed social defects, yet far from those of the srrm3 mutant (blind) fish. 6/9 110 0
