(3/4) The video shows NPCs in a neuron within an expanded volume of ~6.37×4.33×0.2mm (≈25×17µm×0.8 µm PreExM). This was acquired with a 10x air objective but individual NPCs are still visible 😁 At the end we show a particle-averaged reconstruction from ~20 NPCs, with a resolution of ~35 Å!