Riddhiman Garge @riddhiman.bsky.social · 25/04/20264/ We & others have profiled gastruloids extensively with scRNA-seq & imaging. But proteins, (the workhorses of the cell) have been largely uncharted. RNA only modestly predicts protein levels, & misses PTMs entirely. 100
Riddhiman Garge @riddhiman.bsky.social · 25/04/20265/ To fill this gap, we used multi-omics to profile proteins, phosphosites, & RNA (in a experimentally matched fashion) across 4 stages in mouse & human gastruloids. 110
Riddhiman Garge @riddhiman.bsky.social · 25/04/20266/ Quantifying ~7.3K human & ~8.7K mouse proteins, we see thousands of differentially expressed proteins across stages, often in coordinated temporal shifts. e.g., the systematic downregulation of mitochondrial OxPhos proteins over gastruloid development. 100
