iiinova — where your digital self keeps evolving

Replies

Joanna Masel @joannamasel.bsky.social · 12/12/2024

Our annotations of LUCA’s domains agree well with recent annotation of LUCA’s full-length genes by @emoody.bsky.social @phil-donoghue.bsky.social. doi.org/10.1038/s415... Recent gene-tree species-tree reconciliation methods are what made inferring LUCA possible for both groups. 13/15

Table showing that 95 of our pre-LUCA Pfams where deemed by Moody et al. to be >75% likely to be in LUCA proteins, 3 were 50-75% likely, 2 were <50% likely, and 1 was not in their dataset. For our single-copy LUCA Pfams, 672 were confirmed at the >75% level, 75 at the 50-75% level, 106 were <50%, and 15 were not in their dataset.

351

Joanna Masel @joannamasel.bsky.social · 12/12/2024

We had previously found an exceptionally long-term trend: older animal domains have their hydrophobic amino acids more interspersed along the primary sequence elifesciences.org/articles/57347, driven by differential loss rates. We confirmed that this trend goes all the way back to LUCA. 14/15

Schematic of hydrophobic residue clustering vs interspersion, and violin plot showing that LUCA domains have more interspersion than post-LUCA, which have more than modern domains. All are more interspersed than the clustering score of 1 expected by chance.

162

Joanna Masel @joannamasel.bsky.social · 12/12/2024

Spread the word that I have a postdoc opening right now, and am also open to PhD applications evol.mcmaster.ca/brian/evoldi.... We have lots of follow up ideas! 15/15

evol.mcmaster.ca

https://evol.mcmaster.ca/brian/evoldir/PostDocs//UArizona.AminoAcidSubstitutionModels

Postdoc position: amino acid substitution models A postdoc position is available with PI Joanna Masel (https://www.masellab.org/) at the University of Arizona in Tucson. Tucson is a vibrant city of n...

1115