Bret Freudenthal @freudlab.bsky.social · 11/08/20265/ MUTYH needs to engage both strands with its N- and C-terminal domains. Modeling shows it cannot do that in an NCP without clashing into the histones. Steric exclusion, not altered lesion presentation, is the barrier. 100
Bret Freudenthal @freudlab.bsky.social · 11/08/20266/ This fits MUTYH as a post-replicative enzyme working at the fork, and gives a mechanistic explanation for elevated G to T transversions in histone-bound DNA after oxidative stress. 100
Bret Freudenthal @freudlab.bsky.social · 11/08/20267/Wonderful collaboration with Sarah Delaney's lab (Brown), Sheila David's lab (UC Davis), and Emmanuelle Bignon lab (Lorraine), plus cryo-EM support from @CryoEM_PNCC and the KUMC EM facility.static.klipy.comMoira Rose: If That Isn't TeamworkALT: Moira Rose: If That Isn't Teamwork 000
