The sulfonamide motif is a privileged pharmacophore found in numerous therapeutics, and seven-membered cyclic sulfonamides in particular are valued for their structural and biological properties. Howe...

Enzyme design with non-canonical amino acids has achieved rapid progress in biocatalysis. Here, the authors present an efficient strategy for the design of artificial enzymes with unnatural catalytic center by the combination of non-canonical amino acid biosynthesis and genetic incorporation into protein of interest.

The design of artificial enzymes represents a transformative advancement in biocatalysis, enabling the creation of enzyme for nonnatural reactions. Herein, recent progress in the design of enzymes is...