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Reposted by Will Comstock, PhD

Smolka Lab @smolka-lab.bsky.social · 13/11/2025

Check out our new biosensor technology to study DDR kinase signaling: ProKAS. We combine: -proteomics -engineered peptide sensors -a new concept of amino acid barcodes ProKAS tracks kinase signaling with spatial resolution and produces highly quantitative data. Just published today: rdcu.be/ePNo0

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Reposted by Will Comstock, PhD

Mateusz Wagner @mateuszwagner.bsky.social · 05/06/2025

Last day repping @smolka-lab.bsky.social with @wjcomstock.bsky.social at the 73nd(?) #ASMS2025! Had an awesome time—already excited for next year!

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Will Comstock, PhD @wjcomstock.bsky.social · 13/02/2025

My first-author paper on uncovering a non-canonical Tel1 motif using phosphoproteomics has been published in JBC! Amazing to work with @rainshj.bsky.social using both untargeted and targeted mass spectrometry. Check it out: doi.org/10.1016/j.jb...

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Reposted by Will Comstock, PhD

Smolka Lab @smolka-lab.bsky.social · 17/12/2024

Excited to share our preprint reporting a completely new approach to study kinase signaling: ProKAS. ProKAS is based on a tandem array of peptide sensors with barcodes for multiplexed, spatial and kinetic applications. We applied it to DDR kinases. Please share. www.biorxiv.org/content/10.1...

We developed a proteomic kinase activity sensor platform (ProKAS) for the analysis of kinase signaling using mass spectrometry. ProKAS is based on a tandem array of peptide sensors with amino acid barcodes that allow multiplexed analysis for spatial, kinetic, and screening applications.

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Reposted by Will Comstock, PhD

Smolka Lab @smolka-lab.bsky.social · 21/01/2025

In this new paper we find that Tel1 kinase (yeast ATM) targets D/E-S/T motifs… more prevalent than S/T-Q! Mec1 (yeast ATR) can’t target D/E-S/T, so we think D/E-S/T targeting allows Tel1 functional specialization. A mutation in Tel1 makes it only target S/T-Q… and cells become CPT sensitive.

sciencedirect.com

Profiling Tel1 Signaling Reveals a Non-Canonical Motif Targeting DNA Repair and Telomere Control Machineries

The stability of the genome relies on Phosphatidyl Inositol 3-Kinase-related Kinases (PIKKs) that sense DNA damage and trigger elaborate downstream si…

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