Reposted by Weine Kok
Out yesterday. Using a translation reporter on a mini-tn5 transposon, and high freq. mutagenesis, we looked at translation on whole genome scale independently of genome annotation. academic.oup.com/nar/article/...
academic.oup.com
PIRT-Seq: a high-resolution whole-genome assay to identify protein-coding genes
Abstract. The advent of high-density mutagenesis and data-mining studies suggest the existence of further coding potential within bacterial genomes. Small