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Tsai-Hsuan Weng

@thweng.bsky.social

107 followers 176 following 13 posts

Structural biologist @mpibp.bsky.social | Exploring membrane transporter structures with #cryoEM ❄️🔬

Tsai-Hsuan Weng @thweng.bsky.social · 15/06/2026

Together, these data link pH sensing, ion binding, and N-terminal dynamics in NhaA, providing a structural framework for understanding Na⁺/H⁺ antiporters.

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Tsai-Hsuan Weng @thweng.bsky.social · 15/06/2026

We also captured a Na⁺-bound state. The ion sits between Asp163, Asp164, Thr132, and water—giving a clear picture of how NhaA recognizes its substrate.

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Tsai-Hsuan Weng @thweng.bsky.social · 15/06/2026

One of the surprises: we can now see the N-terminal tail. It acts like a plug at the cytoplasmic entrance—blocking access at low pH and moving away as pH increases.

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Tsai-Hsuan Weng @thweng.bsky.social · 15/06/2026

It's finally out now in @natcomms.nature.com! We used cryo-EM + simulations to watch the Na⁺/H⁺ antiporter NhaA in action across a wide range of pH—and uncover how pH, Na⁺ binding, and structural changes are coupled. Read here 🔗 www.nature.com/articles/s41...

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