iiinova — where your digital self keeps evolving

ThEVrapies Lab @thevrapies.bsky.social · 11/08/2026

It has been amazing to have a taste of the incredible wisdom of ancient Chinese herbal remedies. I was honoured to be within a great line up: HailingJin, Gabriella Pocsfalvi, Frederic Zenhausern, Ronelle Roth, Laurianne Simon, among many other outstanding researchers.

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ThEVrapies Lab @thevrapies.bsky.social · 11/08/2026

It has been an honour to be keynote speaker at the 2026 Conference on CHM-EVLP Research and Application in Guangzhou, China. I had the chance to learn how traditional Chinese Medicine is revisited to instruct research in plant-derived vesicles.

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Reposted by ThEVrapies Lab

ISEV Plant EVs Task Force @isevplants.bsky.social · 28/07/2026

👉 Next video 🎥 of our introductory series from the 🌱 Plant EVs Task Force Today, Fernando de la Cuesta, Leader of WG2: Education, Dissemination and Divulgation📺. 💪Ready to deliver expert consulting to any institution or the mass media. 📩Contact us!

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Reposted by ThEVrapies Lab

ISEV Plant EVs Task Force @isevplants.bsky.social · 01/07/2026

🥳 Announcing our survey on the nomenclature proposal drafted by WG1. 📝 We are consulting the whole🪴plant vesicle community to get feedback to set the foundations for a future white paper. 🗞️ 🙏 Please enter the link below 👇 forms.cloud.microsoft/pages/respon...

forms.cloud.microsoft

Microsoft Forms

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Reposted by ThEVrapies Lab

ISEV Plant EVs Task Force @isevplants.bsky.social · 26/06/2026

👉Today we have Carine de Marcos, Leader of the Working Group 1 - Nomenclature📃. We are joining efforts to standardize it and comply with @isev.org 🙏Researchers working on plant vesicles, give us feedback!

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Reposted by ThEVrapies Lab

ISEV Plant EVs Task Force @isevplants.bsky.social · 18/06/2026

📣 We are launching a series of videos 📽️ to introduce our 🌿 Plant EVs Task Force. Our Chair Gabriella Pocsfalvi introducing: objectives, relevance and our commitment with standardization of nomenclature, isolation methods, characterization and reproducibility. Stay tuned for the next videos!

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ThEVrapies Lab @thevrapies.bsky.social · 06/11/2025

Nuestro artículo en JEV, destacado en las noticias de la @uam.es Este estudio nos posiciona como referentes en el campo de las EVs de plantas. Gran trabajo de Miriam Morales Gracias @uccuam.bsky.social www.uam.es/uam/investig...

uam.es

Proponen un catálogo de marcadores para estandarizar el estudio de vesículas extracelulares en plantas

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ThEVrapies Lab @thevrapies.bsky.social · 19/09/2025

Huge thanks to the panel members: Prof. Carmen Montiel Prof. Fernando Vivanco Dr. Gloria Alvarez-Llamas Dr. Milagros Castellanos Prof. Luis Gandía

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ThEVrapies Lab @thevrapies.bsky.social · 19/09/2025

Yesterday Ainara González-Moro defended her PhD Thesis brilliantly. The first PhD formed in the ThEVrapies Lab. A great milestone for the group and an honour to have been your director. You have worked hard, learnt lots and have been fundamental for the group in it's rise.

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ThEVrapies Lab @thevrapies.bsky.social · 07/09/2025

We provide evidence supporting the potential of the following protein families as additional PEV markers: aquaporins, vacuolar-type ATPase complex subunits, fasciclin-like arabinogalactan proteins FLA 10 and FLA13, syntaxins other than PEN1, germin-like proteins and calreticulins

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ThEVrapies Lab @thevrapies.bsky.social · 07/09/2025

Our data shows consistent specificity for current PEV markers from A. thaliana TET8 and PEN1, but these are not robustly in PEVs from other species. Alternative markers need to be explored, aiming to create a broader set of standard PEV identity markers

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ThEVrapies Lab @thevrapies.bsky.social · 07/09/2025

Next, we searched for the appearance of protein families from potential plant EV markers in previous proteomic studies of plant EVs. Conservation analyses throughout the plant and animal taxonomy of representative members of these families were also performed

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ThEVrapies Lab @thevrapies.bsky.social · 07/09/2025

We also analysed protein content of SEC fractions not pooled for isolating EVs and calculated an enrichment degree in EVs. This analysis offered key results to determine marker´s specificity, with some potential EV markers equally present in EVs and the soluble AWF proteome

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ThEVrapies Lab @thevrapies.bsky.social · 07/09/2025

To accomplish this, we have employed a two-step solubilization method to extract proteins from plant EVs for LC-MS/MS analysis, identifying as many as 1145 proteins in A. thaliana and 899 in B. oleracea, detected in all 3 biological replicates assayed

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ThEVrapies Lab @thevrapies.bsky.social · 07/09/2025

We achieved the most profound proteomic analysis of AWF-EVs (genuine EVs) from Arabidopsis and red cabbage, ranking potential markers by enrichment in EVs, species-specific abundance and plant specificity, also comparing with previously data and performing conservation analyses

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ThEVrapies Lab @thevrapies.bsky.social · 07/09/2025

There is urgent need for standardisation of identity markers and evidence of their conservation throughout plant taxa. Hoping this publication helps our efforts in the ISEV Plant Task Force building steps towards rigorous characterisation complying with MISEV. Major findings👇

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ThEVrapies Lab @thevrapies.bsky.social · 24/07/2025

Thanks for the invitation! Inspiring talks and vibrant networking. Great hosting @fafianlabora.bsky.social!

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