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David Gally

@strain9000.bsky.social
114 followers 31 following 13 posts

E. coli centric microbiologist now heavily into predicting phage efficacy for UTI in dogs & humans plus O157 in cattle using genomes, interaction datasets and machine learning. Have always loved how bacteria generate heterogeneity…..it’s the spice of life.

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Reposted by David Gally
Onisillos @onisillos.bsky.social · 05/03/2024
New review article in The Lancet Microbe Safety and efficacy of #phage application in bacterial decolonisation: a systematic review www.thelancet.com/journals/lan... #bacteriophage #OpenAccess #OA
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Reposted by David Gally
Andrew Singer @oxonandrew.bsky.social · 06/03/2024
Super interesting study on the use of phage in the environmental surveillance of Salmonella Typhi journals.plos.org/plosntds/art...
journals.plos.org
Detection of Salmonella Typhi bacteriophages in surface waters as a scalable approach to environmental surveillance
Author summary Typhoid fever is a major health issue in low- and middle-income countries, causing illness and death. The World Health Organization recommends the use of typhoid-conjugate vaccines to c...
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David Gally @strain9000.bsky.social · 12/03/2024
Smart stuff, we have been looking at scavenging systems and signalling in E. coli with respect to phage sensitivity but this is nice & specific… doi.org/10.1038/s414...
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Reposted by David Gally
Derek Lowe @dereklowe.bsky.social · 08/01/2024
The links between Epstein-Barr virus infection and later multiple sclerosis are getting stronger: www.science.org/content/blog...
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David Gally @strain9000.bsky.social · 02/01/2024
www.theguardian.com/commentisfre...
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David Gally @strain9000.bsky.social · 18/12/2023
Question on plagiarism software. We use Turnitin at our university but Food Standards Scotland are interested in using something like this but Turnitin very expensive. Does anyone have experience with other software? What do the journals use?
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Reposted by David Gally
Brian Klaas @brianklaas.bsky.social · 13/12/2023
Re: the UK government’s new visa rules, I’ve written about my own experience—spending more than £13,000 on visas and a ridiculous test that had incorrect answers—but also why I still love the UK, even as the government tried to deter me from living here. www.forkingpaths.co/p/my-life-in...
forkingpaths.co
My "Life in the UK" Test and a Great British Travel Guide
I passed my "Life in the UK" test and will soon (hopefully) become a dual citizen of the US and UK. I reflect on 12 years in Britain and offer some less obvious UK travel suggestions for you to enjoy.
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Reposted by David Gally
Vivek Mutalik @vivekmutalik.bsky.social · 04/12/2023
Thrilled to see our Phage CRISPRi paper in @PLOSbiology doi.org/10.1371/jour...
doi.org
Systematic and scalable genome-wide essentiality mapping to identify nonessential genes in phages
In this work, authors use an arrayed genome-wide CRISPR interference assay to map gene essentiality landscape in the canonical coliphages λ and P1.
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David Gally @strain9000.bsky.social · 24/11/2023
So following up on Aude Bernheim’s preprint yesterday, we uploaded our related work on predictive phage therapy for uropathogenic E. coli. Our phage interactions for the predictive models are >9000 interactions in artificial urine…would be great to add more generalist phages…
biorxiv.org
Predictive phage therapy for Escherichia coli urinary tract infections: cocktail selection for thera...
bioRxiv - the preprint server for biology, operated by Cold Spring Harbor Laboratory, a research and educational institution
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David Gally @strain9000.bsky.social · 22/11/2023
Viruses of Microbes - UK (VoM - UK) 25th - 26th January 2024 www.northumbria.ac.uk/about-us/new...
northumbria.ac.uk
Viruses of Microbes (VoM UK) Conference 2024
Viruses of Microbes (VoM UK) Conference 2024- The aim of the meeting is to be a way of bringing the UK Viruses of Microbes community together, focussed at supporting the development of early careers r...
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David Gally @strain9000.bsky.social · 21/11/2023
A heritable iron memory enables decision-making in Escherichia coli
doi.org
PNAS
Proceedings of the National Academy of Sciences (PNAS), a peer reviewed journal of the National Academy of Sciences (NAS) - an authoritative source of high-impact, original research that broadly spans...
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David Gally @strain9000.bsky.social · 09/11/2023
Going back to methods to generate heterogeneity - I always thought this paper by JJ Collins was overlooked (even though in Nature) - you just need the right interacting regulatory systems to generate a biphasic population: rdcu.be/dqEOZ
doi.org
Construction of a genetic toggle switch in Escherichia coli - Nature
Nature - Construction of a genetic toggle switch in Escherichia coli
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David Gally @strain9000.bsky.social · 09/11/2023
Similar can be achieved by subsets that express colanic acid that limits many phage interactions. We are looking at the interplay between levels of O157 antigen, Gp4 capsule and colanic acid expression for phage resistance in vivo. Basis to heterogeneity still to be determined……
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