Reposted by @somiealo.bsky.socialRajendra Singh Negi @dr-rajendra-s-negi.bsky.social · 28/04/2026(7/n) E16 cells showed stronger perpendicular long axis alignment than E14 cells. This suggests that the later mechanical barrier is not sufficient to suppress perpendicular divisions. 121
Reposted by @somiealo.bsky.socialRajendra Singh Negi @dr-rajendra-s-negi.bsky.social · 28/04/2026(10/n) Key results: stratification increases continuously with division angle. Intermediate angles contribute partially to stratification. 111
Reposted by @somiealo.bsky.socialRajendra Singh Negi @dr-rajendra-s-negi.bsky.social · 28/04/2026(12/n) Division rate also matters. Fast divisions act like active mechanical fluctuations, increasing basal-layer rearrangements and driving a fluidization. 111
Reposted by @somiealo.bsky.socialRajendra Singh Negi @dr-rajendra-s-negi.bsky.social · 28/04/2026(n/n) Together, our results suggest a mechanical feedback loop, division orientation controls stratification and basal-layer fluidity, while the developmental mechanical environment tunes how effective each division is. 021
Reposted by @somiealo.bsky.socialRajendra Singh Negi @dr-rajendra-s-negi.bsky.social · 28/04/2026(14/n) Finally, we find that the in plane 2D shape index is a better readout of basal-layer fluidization than the full 3D shape index. In layered tissues, the relevant mechanics are often effectively 2D 121
Reposted by @somiealo.bsky.socialRajendra Singh Negi @dr-rajendra-s-negi.bsky.social · 28/04/2026(9/n) Fate-based stratification: A basal cell changes identity (basal to suprabasal mechanics), which actively drives upward movement. We use this to maintain constant basal density (homeostasis) 111
Reposted by @somiealo.bsky.socialRajendra Singh Negi @dr-rajendra-s-negi.bsky.social · 28/04/2026(8/n) We define two types of stratification. Homotypic stratification: A basal cell moves upward purely due to mechanics 111
Reposted by @somiealo.bsky.socialRajendra Singh Negi @dr-rajendra-s-negi.bsky.social · 28/04/2026(5/n) Our 3D vertex model represents the epidermis as three layers: basement membrane, basal stem-cell layer, and suprabasal layer. We then prescribe the division angle and measure how cells stratify and how the basal layer rearranges 121
Reposted by @somiealo.bsky.socialRajendra Singh Negi @dr-rajendra-s-negi.bsky.social · 28/04/2026(2/n) The epidermis starts as a single layer of progenitor cells and develops into a multilayered barrier. This requires coordinated proliferation, differentiation, delamination, and mechanics. cshperspectives.cshlp.org/content/10/1... 111
Reposted by @somiealo.bsky.socialRajendra Singh Negi @dr-rajendra-s-negi.bsky.social · 28/04/2026(1/n) Does basal stem cell division orientation regulate skin stratification and tissue mechanics? And can tissue mechanics feed back to control division orientation? In our new preprint, we use a 3D vertex model to explore this @manningresearch.bsky.social @somiealo.bsky.socialbiorxiv.org 1105
Reposted by @somiealo.bsky.socialManning Research Group @manningresearch.bsky.social · 10/02/2026Excited to highlight a new preprint about mechanical contributions to tissue homeostasis, from the Manning group in collaboration with the amazing Carien Niessen and Sara Wickstrom @sarawickstrom.bsky.social labs, spearheaded by Dr. Somiealo Azote: www.biorxiv.org/content/10.6... 1339
