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Shasha Chong

@schong-ustc.bsky.social

Assistant Professor of Chemistry at Caltech studying gene regulation, intrinsically disordered proteins, biomolecular condensates, and cancer

Posts Replies Media

Shasha Chong @schong-ustc.bsky.social · 16/07/2026

(5/5) Our results reveal new insights into the assembly and regulation of endogenous transcription factor hubs. Warm congratulations to Shawn Yoshida and Yanghao Zhong in my lab on the new publication!🎉🎉🎉 Thanks to our collaborators Bill Dunphy and Akiko Kumagai for their generous support!

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Shasha Chong @schong-ustc.bsky.social · 16/07/2026

(4/5) Nascent RNA destabilizes EWS::FLI1 hubs without affecting their dimensions. LY2835219 and trabectedin, compounds previously reported to affect EWS::FLI1 function, disrupt and mislocalize endogenous EWS::FLI1 hubs, respectively, highlighting their therapeutic potential.

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Shasha Chong @schong-ustc.bsky.social · 16/07/2026

(3/5) Hub formation is a neomorphic behavior of EWS::FLI1 not conferred by its parental proteins, EWSR1 and FLI1. During mitosis, EWS::FLI1 hubs dissolve, but EWS::FLI1 molecules continue to dynamically associate with mitotic chromosomes.

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Shasha Chong @schong-ustc.bsky.social · 16/07/2026

(2/5) We characterized the formation and regulation of endogenous EWS::FLI1 hubs in their native cellular context at the single-molecule level. We showed that the hubs are sub-diffraction-limit objects with dissolution mechanisms that prevent macroscopic phase separation.

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Shasha Chong @schong-ustc.bsky.social · 16/07/2026

(1/5) Excited to share our new paper in Advanced Science! We combine genome editing, super-resolution microscopy, and single-molecule tracking to investigate the endogenous oncogenic fusion transcription factor EWS::FLI1 in Ewing sarcoma cells. advanced.onlinelibrary.wiley.com/doi/full/10....

advanced.onlinelibrary.wiley.com

Dynamic Regulation of Endogenous Transcription Factor Hubs at Single‐Molecule Resolution

This study combines single-molecule microscopy and genome editing to characterize the dynamic behaviors of endogenous oncofusion transcription factor EWS::FLI1 in Ewing sarcoma cells. EWS::FLI1 forms...

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