I recently built PolySNiP-nf because standard CRISPR tools failed in polyploids. When a colleague’s octoploid strawberry data showed 30% "missing" reads and false chimera calls, the issue wasn't the sequencing, it was the diploid assumptions built into the software.
github.com/rj-price/Pol...
github.com
GitHub - rj-price/PolySNiP-nf: Nextflow pipeline for polyploid CRISPR amplicon genotyping
Nextflow pipeline for polyploid CRISPR amplicon genotyping - rj-price/PolySNiP-nf