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Rich Davis

@richdavisphd.bsky.social

1.3K followers 263 following 2 posts

Clinical Microbiology Lab Director. Sharing clinical micro pics with #MicroRounds (originally on another social media site); he/him

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Rich Davis @richdavisphd.bsky.social · 09/08/2023

#MicroRounds (Day 977): "day at the beach" For a lab to conclusively detect beta-lactamase in Staphylococcus spp. this is the zone-edge test (aka "sandy beach test"). Around a penicillin disk a sharp ("cliff"): beta-lac(+). A fuzzy edge ("beach"): (-) #ASMClinMicro #IDTwitter

A side by side image of a bacterial growth media with white antibiotic disks and zones around them. On the left a zone with a sharp edge, onthe right a zone with a hazy, fuzzy edge

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Rich Davis @richdavisphd.bsky.social · 08/08/2023

#MicroRounds (Day 976): "Home sweet holmesii" A respiratory culture on charcoal Regan-Lowe medium (supports Bordetella growth, neutralizes toxic compounds): Bordetella holmesii. Silvery colonies. In PCR, B. holmesii carries IS481 (like B. pertussis) #ASMClinMicro #IDTwitter

A black bacterial culture media with a lot of bacterial colonies. Caption reads, Bordetella holmesii
Respiratory culture – other flora present
Regan-Lowe agar: charcoal based – horse blood; charcoal neutralizes fatty acids, peroxides; AmpB, cephalexin
Silvery, pearly colonies
PCR: carries insertion sequence IS481 (like B. pertussis, but fewer copies than B. pertussis)

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