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Mikhail Pogorelyy

@pogorely.bsky.social
63 followers 66 following 4 posts

Immunologist @ FredHutch. TCR repertoires, virology, algorithms

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Reposted by Mikhail Pogorelyy
Paul Thomas @pgtimmune.bsky.social · 04/09/2026
TCR specificity prediction is advancing rapidly. The central question is not only which TCR sequence recognizes an antigen, but also which TCR pairings make recognition possible & where is the binding boundary? Our new paper tackles these questions. 🧵 www.science.org/doi/10.1126/...
science.org
Uneven TCR chain pairing constraints govern epitope recognition
Combinatorial pairing of independently recombined T cell receptor (TCR) α- and β-chains is central to diversifying the TCR repertoire. Although sequence motifs in one chain correlate with epitope reco...
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Mikhail Pogorelyy @pogorely.bsky.social · 25/11/2025
Code is on the Github github.com/pogorely/TIRTL, and there is a new R package with more TIRTL-seq data analysis and visualization functions (actively developed by @nick-clark-bioinfo.bsky.social), early version is ready for testing github.com/NicholasClar...! Congrats to TIRTL team!(4/4)
github.com
GitHub - pogorely/TIRTL: Code for paired TCR data analysis from TIRTL-seq experiments
Code for paired TCR data analysis from TIRTL-seq experiments - pogorely/TIRTL
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Mikhail Pogorelyy @pogorely.bsky.social · 25/11/2025
Fully manual 96-well protocol is also at protocols.io: www.protocols.io/view/tirtl-s.... Requires only manual multichannel pipettes, no specialised equipment! (3/4)
protocols.io
Bring structure to your research - protocols.io
A secure platform for developing and sharing reproducible methods.
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Mikhail Pogorelyy @pogorely.bsky.social · 25/11/2025
Detailed 384-well protocol is at protocols.io: www.protocols.io/view/tirtl-s... (2/4)
protocols.io
Bring structure to your research - protocols.io
A secure platform for developing and sharing reproducible methods.
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Mikhail Pogorelyy @pogorely.bsky.social · 25/11/2025
Excited to see our TIRTL-seq method published in Nature Methods www.nature.com/articles/s41...! Some updates after the revision: (1/4)
nature.com
TIRTL-seq: deep, quantitative and affordable paired TCR repertoire sequencing - Nature Methods
TIRTL-seq is a high-throughput method for paired T cell receptor sequencing at the cohort scale.
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Reposted by Mikhail Pogorelyy
Paul Thomas @pgtimmune.bsky.social · 24/11/2025
Two complementary studies from our group out today: a therapeutic peptide vaccine trial for fibrolamellar carcinoma (FLC) & TIRTL-Seq, our robust method for deep quantitative paired TCR sequencing a🧵w/ @markyarchoan.bsky.social & @pogorely.bsky.social www.nature.com/articles/s41...
nature.com
TIRTL-seq: deep, quantitative and affordable paired TCR repertoire sequencing - Nature Methods
TIRTL-seq is a high-throughput method for paired T cell receptor sequencing at the cohort scale.
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