The detoxified E. coli strain ClearColi enables high-yield phage production while simplifying the critical process of removing endotoxins.

Using the detoxified ClearColi strain for phage production maintains yields while simplifying the critical process of removing endotoxins.

Using the detoxified ClearColi strain as a host simplifies phage purification by reducing endotoxins without compromising viral yield.

Using the detoxified ClearColi strain for phage production maintains yields while simplifying the removal of dangerous endotoxins.

Combining peptides, antibiotics, and phages completely clears multidrug-resistant Serratia marcescens biofilms.

The survival of pathogenic microorganisms on surfaces is contingent on environmental factors, including humidity and temperature. In order to analyze the survival and persistence in vitro, different protocols have already been implemented. This has resulted in a wide range of survival rates of all k …

Chronic wound infections caused by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) often refractory to conventional therapies due to bacterial drug resistance and biofilm formation. To address this challenge, we developed a synergistic antibacterial nanoarchitectonics integrating bacteriop …

The emergence and global spread of New Delhi metallo-β-lactamase-1 (NDM-1) pose a severe threat to the efficacy of carbapenems, which are considered the last line of defense against infections caused by multidrug-resistant gram-negative bacteria. In this study, through virtual screening and enzyme a …

The polymicrobial infections caused by Vibrio species, especially multidrug-resistant isolates, are posing increasing threats to the health of coastal residents. Herein, a novel dodecapeptide, denoted as D-zp37, was designed and synthesized, aiming to combat these notorious pathogens. Experimental r …

Mycobacteriophage MP2445 exhibits siphovirus morphology and infects <i>Mycobacterium smegmatis</i>, <i>Mycobacterium bovis</i> BCG, and <i>Mycobacterium fortuitum</i>. Its genome is 50,711 bp in length, has a GC content of 63.34%, and contains 78 protein-coding sequences and one tRNA. MP2445 shares …

The survival of pathogenic microorganisms on surfaces is contingent on environmental factors, including humidity and temperature. In order to analyze the survival and persistence in vitro, different protocols have already been implemented. This has resulted in a wide range of survival rates of all k …

A triple-combo of peptides, antibiotics, and phages completely wiped out MDR Serratia marcescens biofilms.

A triple combo of peptides, antibiotics, and phages completely wipes out MDR Serratia marcescens biofilms.

Comprehensive characterization of mammalian gut viromes is essential for early detection of commensal and potentially zoonotic viruses and for reducing the risk of cross-species transmission. Viral metagenomics was applied to profile gut viral communities from zoo mammals maintained across multiple …

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Chronic wound infections caused by methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) often refractory to conventional therapies due to bacterial drug resistance and biofilm formation. To address this challenge, we developed a synergistic antibacterial nanoarchitectonics integrating bacteriop …

Conventional cancer therapies, such as radiotherapy, chemotherapy, and surgery, exhibit notable limitations in the treatment of solid tumors. Bacteria-mediated tumor therapy has been proven to be effective in clinical studies and is regarded as a promising strategy for tumor treatment. <i>Salmonella</i> …

Bacteriophages, or phages, are the viruses of bacteria. Since at least the later 1940s, researchers have studied phages using spectrophotometrically determined measures of bacterial-culture turbidity (optical density). Recently, two groups have proposed the use of kinetic visualizations of phage-ind …

To remove viruses from water, the use of self-assembling liquid crystals is presented as a novel method for the synthesis of membranes with a regular pore size (below 1 nm) and controlled pore structures. Nanostructured bicontinuous cubic liquid-crystalline (LC) thin films are photopolymerized onto …

Viruses are crucial components of microbial communities, both phage that infect bacterial community members as well as pathogenic and other eukaryotic viruses. However, they remain unobserved by most current technologies, due to combinations of experimental and analytical factors. To address the lat …

Phage therapy has attracted increasing attention as a potential antibacterial strategy. Phage virion proteins (PVPs) are vital for recognizing host cells and binding to their surface receptors. Thus, accurate PVP identification is essential for developing antibacterial agents. In this study, we intr …

The scarab species <i>Protaetia brevitarsis</i>, an edible insect, has been used in traditional medicine, as animal feed, and for converting agricultural organic wastes into biofertilizer. The intestinal tract, which contains a diverse array of microbiota, including viruses, plays a critical role in …

Bacteriophages (phages) are viruses that target bacteria and hold great promise as therapeutics against multidrug-resistant bacteria, pathogen infections, and for microbiota engineering. Despite their potential, the effect of phage therapy on gut microbiota remains insufficiently explored. This stud …

Bacterial infections continue to persist as a major global healthcare challenge, exacerbated by the alarming rise of antimicrobial resistance (AMR) that renders conventional antibiotics increasingly ineffective. In response, bacteriophage therapy has re-emerged as a viable alternative to antibiotics …

Phage display is a powerful technology for identifying new ligands, including mineral-selective peptides. However, it is prone to several biases that may lead to false positives or false negatives, particularly due to non-specific binding, amplification bias, and the limited number of clones typical …

Cryo-electron microscopy (cryo-EM) has revolutionized structural virology, enabling routine structure determination at 2-4 Å resolution, with exceptional cases reaching 1.56 Å. The structural diversity of viruses across vertebrate, plant, and insect hosts provides fundamental insights into infection …

Phages dominate the human gut virome and are known for their ability to prey on bacteria and shape microbiota. However, their response to diet has only been elucidated using small-scale studies. By integrating a massive meta-analysis of 6932 diet-associated metagenomes with a time-resolved mouse mod …

Collectively, these findings provide novel insights into the gut microbial factors regulating FE in ducks, offering potential strategies to optimize poultry nutrition and productivity. Video Abstract.

Multi-drug resistant bacteria (MDR) pose a major public health challenge. Their ability to exchange resistance genes through Horizontal Gene Transfer (HGT) promotes the appearance of resistant strains, limiting antibiotic treatments for infections caused by these MDR bacteria. Among alternative appr …

Subretinal injection remains the standard method for delivering genes to the retina. However, diffusion into deeper retinal layers is often limited by sequestration of wild-type AAV2 vectors at the retinal pigment epithelium (RPE). In this study, we used in vitro phage display strategy to identify n …

Colibactin is a bacterial genotoxin that is linked to colorectal cancer and implicated in interbacterial competition; however, its role in natural communities remains unknown. <i>Frischella perrara</i>, a symbiont living only in honeybee guts, produces colibactin and causes DNA damage. Here, we foun …

Virus-prokaryote interactions within microbial aggregates critically influence microbiome function and stability, yet the interactive dynamics during microbial aggregation remain largely unexplored. Here, longitudinal multi-omics revealed that prokaryotic host community diversity underwent decline a …

The emergence of multidrug-resistant Enterobacter cloacae complex (ECC) strains, particularly Enterobacter hormaechei, underscores the urgent need for alternative antimicrobial strategies. This study reports the isolation and comprehensive characterization of three virulent bacteriophages - Enteroba …

CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) systems present a barrier to prophage acquisition by restricting invading phages or by inducing autoimmune cleavage of integrated prophage DNA. The RNA-sensing type VI CRISPR nuclease Cas13 mediates …

P2-like viruses (P2Vs), temperate phages of the Peduoviridae family, possess a lysogenic lifestyle that renders traditional isolation unsuitable, hindering our understanding of their diversity and ecological roles. Utilizing profile-hidden Markov models to analyze public databases, this study achiev …

The xylem-limited bacterium Xylella fastidiosa (Xf) is increasingly threatening global agriculture due to the absence of effective control strategies. Phage biocontrol represents a promising alternative; however, only a limited number of bacteriophages have been identified against Xf. In this study, …

Toxin-antitoxin-chaperone (TAC) systems are three-part gene clusters encoding a toxin, antitoxin, and specialized SecB-like chaperone (SecB^TA) with emerging roles in phage defense. To identify and classify SecB homologs and associated TACs across bacteria, we surveyed the full RefSeq data …

Bacteriophages (phages) play a critical role in controlling bacterial populations, both in nature and as potential therapeutic agents. Their ability to replicate, compete against each other, and eradicate target cell populations is usually understood through a number of 'life history parameters', tr …

These studies enrich our understanding of DNA phage dynamics in gut while emphasizing the predominance of "piggyback-the-winner" strategies.

Lytic phages have emerged as promising candidates for feed additives for controlling <i>Salmonella</i> in poultry, owing to their high specificity, self-replication, and excellent stability. According to the European Food Safety Authority (EFSA) guidelines, their application as feed additives requir …