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Peter Dougherty

@peterdoug.bsky.social
74 followers 14 following 11 posts

PhD student at the University of Copenhagen, interested in microbiology and bioinformatics

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Peter Dougherty @peterdoug.bsky.social · 02/01/2025
If these phages are non-temperate, how do they persist in bacterial cultures? Given their diversity, we argue there is not a single explanation. Here we suggest three possible mechanisms for persistence: phase variation, asymmetric phage inheritance, and lysis inhibition.
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Peter Dougherty @peterdoug.bsky.social · 02/01/2025
In a targeted follow-up search for the three jumbo-phage clusters in bacterial assemblies beyond Escherichia, we found an additional 285 persistent jumbo-phages. The Seoul- and Goslar-like phages encoded chimallin, a protein some phages use to produce a protective phage nucleus.
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Peter Dougherty @peterdoug.bsky.social · 02/01/2025
We also clustered the persistent phages with all virulent Escherichia phage isolates. Most (27/37) clusters contained persistent phages, demonstrating this is broad phenomenon. However, the three clusters with a highest proportion of persistent phages were all jumbo phages.
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Peter Dougherty @peterdoug.bsky.social · 02/01/2025
After extensive QC, we found 373 non-temperate phage genomes. These phages were not integrated in the bacterial genome, and do not resemble phage-plasmids.
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