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pauldatlinger.bsky.social

@pauldatlinger.bsky.social

pauldatlinger.bsky.social @pauldatlinger.bsky.social · 24/09/2025

#CELLFIE for CAR T screening—a new mRNA-based platform for screening primary cells. CAR + gRNA library are delivered by lentivirus, CRISPR modifiers as electroporated mRNA. That’s more flexible and effective than existing T cell screening methods. (1/7)

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pauldatlinger.bsky.social @pauldatlinger.bsky.social · 24/09/2025

CAR T cells showcase the enormous potential of cell therapies, but often fail due to lack of evolutionary optimization. Today in @nature.com , we use #CELLFIE to engineer cell therapies at scale and share the largest resource of CRISPR screens in CAR T cells. www.nature.com/articles/s41...

nature.com

Systematic discovery of CRISPR-boosted CAR T cell immunotherapies - Nature

CELLFIE, a CRISPR platform for optimizing cell-based immunotherapies, identifies gene knockouts that enhance CAR T cell efficacy using in vitro and in vivo screens.

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