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Olivia Hazelwood

@ohazel.bsky.social
95 followers 55 following 15 posts

Researching plant cellular and developmental biology at the Ashraf Lab of UBC. @corylus_L on X

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Olivia Hazelwood @ohazel.bsky.social · 09/01/2025
I am happy to share that I've began my PhD program with @aribidopsis.bsky.social at @ubcbotany.bsky.social ! 😊🌱
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Olivia Hazelwood @ohazel.bsky.social · 12/12/2024
(5/5) – We would like to give a very special thanks to @minyaaa.bsky.social for inviting us to write for this special issue! We are also very happy and grateful that this paper was handled by Minako Ueda sensei! Article: doi.org/10.1017/qpb.... Lab website: www.ashraflab.com/publications...
the Ashraf Lab!!!
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Olivia Hazelwood @ohazel.bsky.social · 12/12/2024
(4/5) – But there is still always room for improvement in plant cell cycle markers. We proposed the combination of two existing marker lines (PCNA-GFP and tubulin marker) to visualize all stages of the cell cycle, including each stage of mitosis.
Proposed plant cell cycle marker line combining proAtPCNA1::AtPCNA1-mNeonGreen and proUBQ10::mScarlet-TUB6 to visualize distinct cell cycle stages (G1, early S, late S, G2, prophase, metaphase, anaphase and telophase) and mitotic structures
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Olivia Hazelwood @ohazel.bsky.social · 12/12/2024
(3/5) – Luckily, plant cell biologists have become very creative to circumvent the challenges of developing a FUCCI equivalent marker. This figure summarizes the four markers that have helped visualize each stage of the cell cycle so far. Feel free to use this illustration.
Visualization of plant cell cycle marker lines (CYCB1;1-GUS, Cytrap, proAtPCNA1::AtPCNA1-sGFP and PlaCCI) throughout the distinct cell cycle stages (G1, early S, late S, G2, prophase, metaphase, anaphase and telophase).
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Olivia Hazelwood @ohazel.bsky.social · 12/12/2024
(2/5) – You may already know that cell cycle marker lines have progressed much further in animals compared to plants. But why? It’s because in plants, there are challenges associated with developing a FUCCI equivalent marker. We go into more detail regarding this in the review.
Graphical summary of cell cycle marker lines and each stage they visualize
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Olivia Hazelwood @ohazel.bsky.social · 12/12/2024
(1/5) – I am very excited to share our publication in QPB with @aribidopsis.bsky.social ! Before working with Arif, I thought I knew at least the basics about the plant cell cycle, but shortly I realized that I knew almost nothing. If this sounds relatable at all, then this review is for you!
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Olivia Hazelwood @ohazel.bsky.social · 19/11/2024
Yesterday was our last Microscopy Monday of the fall semester! We were very thankful and lucky to have @fisherke.bsky.social lead a wonderful session covering considerations for electron microscopy 🔬🦠🩷 #Microscopy_Monday_Howard_University
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