Thank you to all who attended the 2026 7th Bioelectronic Medicine Summit!
Stay tuned for poster winners, meeting report, and compiled poster abstracts.
(Meeting organizers, co-chairs, left to right: Dr. Yousef Al-Abed, Dr. Eric H. Chang, Dr. Christopher Puleo, Dr. Kevin J. Tracey)
![Figure 1. Schematic overview of the experimental design for cell and mouse studies.
Schematic representation of the integrated cell- and mouse-based experimental design used to investigate the role of GNMT in modulating methotrexate (MTX)-induced metabolic remodeling. In cell-based studies, liver-derived cell lines with or without GNMT expression were treated with MTX and subjected to stable isotope tracer analyses to assess mitochondrial one-carbon metabolism, glycine cleavage system (GCS)
activity, and central carbon metabolic pathways. In parallel, wild-type (Gnmt⁺/⁺) and GNMT knockout (Gnmt⁻/⁻) mice were administered low-dose MTX or vehicle control over a long-term treatment period. Mice
were subsequently fed defined diets containing stable isotope tracers, including L-[2,3,3-²H₃] serine, or [2¹³C] glycine, to enable in vivo metabolic tracing. Bone marrow, liver, and plasma samples were collected to quantify isotopic enrichments in folate-mediated one-carbon metabolites, deoxynucleotide synthesis, DNA methylation, glycolysis, and TCA cycle intermediates using gas chromatography–mass spectrometry (GC–MS).](https://cdn.bsky.app/img/feed_thumbnail/plain/did:plc:rzadxzztkeptiemot5shx6zy/bafkreibfmsm7dpfiuby464dx3nsutguxjcrw7ef6s6rjtb33ys47yxispu)
