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Netanya Bernitz

@netanyabernitz.bsky.social

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Senior Laboratory Research Scientist in the Cryptosporidiosis Laboratory at The Francis Crick Institute, London

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Reposted by Netanya Bernitz

bishara marzook @ghostpathogen.bsky.social · 24/07/2025

Our paper has finally graduated from pre-print to peer-reviewed, pretty much unscathed, and is out now! www.cell.com/cell/fulltex...

cell.com

The essential host genome for Cryptosporidium survival exposes metabolic dependencies that can be leveraged for treatment

An arrayed microscopy-based CRISPR screen revealed host genes affecting multiple infection phenotypes of the intracellular parasite Cryptosporidium. Hits in the host cholesterol biosynthesis pathway a...

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Reposted by Netanya Bernitz

bishara marzook @ghostpathogen.bsky.social · 06/12/2024

Have you ever wondered how knocking out every single gene in the human genome, one by one, might affect your intracellular pathogen of interest? We did, & boy was it a wild ride! Happy to share our preprint discovering the essential host genome for Cryptosporidium - www.biorxiv.org/content/10.1...

Schematic showing the experimental outline for a full genome arrayed CRISPR screen. 384-well plates containing cells with an arrayed crRNA library are infected with Cryptosporidium parasites, which undergo asexual rounds of replication intracellularly, followed by sexual development to male and female forms. Plates are then fixed and immunolabeled to detect all parasites, female parasites, host actin recruited to parasite vacuoles, and host nuclei. Three z-stacks were imaged by high content microscopy to detect apically located parasites as well as host cell actin and nuclei.

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