iiinova — where your digital self keeps evolving

Mathias Mahn

@mmahn.bsky.social

756 followers 389 following 8 posts

Trying to bridge cellular, circuit, and behavior neuroscience - from designing molecular tools to studying complex behaviors.

Posts Replies Media

Mathias Mahn @mmahn.bsky.social · 17/12/2024

Please share: the FMI is hiring a tenure-track Group Leader in Multicellular Systems to explore the molecular and cellular mechanisms driving the formation, maintenance, or destabilization of tissues, organs and organisms. Apply at: www.fmi.ch/education-ca...

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Reposted by Mathias Mahn

Nikolas Karalis @nikolaskaralis.bsky.social · 17/11/2024

How to rebuild your network on @bsky.app and improve your experience here? I made a short starter guide with resources. www.neuronaldynamics.eu/resources/bl... Also, a thread 🧵 below.

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Reposted by Mathias Mahn

Ofer Yizhar @oyiz.bsky.social · 28/11/2024

Many people have asked me what's the deal with "SIO" in our Addgene vectors (www.addgene.org/Ofer_Yizhar/) - we use these in most of our Cre-dependent AAVs - so here's a brief explanation of how it works.

addgene.org

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Mathias Mahn @mmahn.bsky.social · 29/11/2024

Thanks for the shout out :)

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Mathias Mahn @mmahn.bsky.social · 29/11/2024

Lutz's lab showed that directional lox sites work in vertebrate cells. Originally, the generation of the mutant lox sites was done by Albert et al. 1995: doi.org/10.1046/j.13... to allow for the integration of a single gene copy into the plant chromosome.

doi.org

Site‐specific integration of DNA into wild‐type and mutant lox sites placed in the plant genome

The bacteriophage P1 Cre—lox site-specific recombination system has been used to integrate DNA specifically at lox sites previously placed in the tobacco genome. As integrated molecules flanked by wi....

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Mathias Mahn @mmahn.bsky.social · 29/11/2024

One difference is that the DIO approach leads to the permanent removal of DNA, making it irreversible. For SIO an equilibrium between forward and reverse recombinantion is created. However the reverse probability is low, creating a bias to the activated state. In practice expression levels are good.

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Mathias Mahn @mmahn.bsky.social · 21/11/2024

Let me in please 🙏

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Mathias Mahn @mmahn.bsky.social · 17/11/2024

stGtACR & eOPN3

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Mathias Mahn @mmahn.bsky.social · 17/11/2024

Got one trangene expressing IPSC clone after weeks of trying, finally grew enough to split it - sucked it right into the liquid waste 🙈

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Mathias Mahn @mmahn.bsky.social · 13/11/2024

Do you want to be my "new sciencetwitter" friend? 🤗

Broken Twitter bird with x-ed out eyes. Butterfly emerging from the wreckage.

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