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MIDORI 2

@midorireference.bsky.social

Reference database for taxonomic assignment of eukaryotic mitochondrial DNA sequences. Available in 9 formats: RDP, MOTHUR, QIIME, SPINGO, SINTAX, DADA2, KRAKEN2, and BLAST+. reference-midori.info #bioinformatics #eDNA #metabarcoding

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MIDORI 2 @midorireference.bsky.social · 12/09/2026

Making MIDORI2 FAIR: findable, accessible, interoperable, and reusable reference databases The new GB273 release includes nine file formats, including the KRAKEN2 format. All future releases will be deposited on Zenodo to support FAIR practices. zenodo.org/records/2268...

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MIDORI 2 @midorireference.bsky.social · 25/08/2026

MIDORI2 reference databases support the following: 🔹 LONGEST: compact, representative sequences for efficient taxonomic assignment. 🔸 UNIQ: distinct sequence variants and haplotypes for greater intraspecific diversity. Choose based on your research question and taxonomic coverage.

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