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Michael Glickman

@michaelglickman.bsky.social

1.7K followers 541 following 123 posts

Physician Scientist interested in all things mycobacterial: pathogen, model system, and cancer therapy. Immunology program/ID MSK. Opinions are mine and do not represent those of employer, MSK. Pubs: shorturl.at/GaVRW

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Michael Glickman @michaelglickman.bsky.social · 19/01/2026

I saw this incredible quilt at the Renwick gallery at the Smithsonian so I thought I would link it to the only quilting person I have seen. It documents the daily temperature of a year by color. There is a deeper key that was not posted I believe.

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Michael Glickman @michaelglickman.bsky.social · 28/07/2025

We show that these iPSC derived alveolar macrophages, which we call AM-L (like), resemble bonafide AMs at baseline and with Mtb infection, leveraging the insights of our collaborators in the Hawn lab published here: journals.aai.org/jimmunol/art...

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Michael Glickman @michaelglickman.bsky.social · 28/07/2025

To faciliate human AM studies, we derived macrophage subtypes from iPSCs under distinct cytokines, including GM-CSF, which is required for development of AMs.

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Michael Glickman @michaelglickman.bsky.social · 25/03/2025

And also show that placing the ALFA tag at different places in a multidomain protein (Pks13) allows for domain specific interactomes:

Venn diagram of proximity hits with the ALFA tag at different positions in Pks13

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Michael Glickman @michaelglickman.bsky.social · 25/03/2025

And can be used for proximity proteomics by fusing NBALFA to TurboID. We present a full workflow for proximity proteomics in M. smegmatis and M. tuberculosis. Here shown for the mycolic acid biosynthetic protein Pks13:

Volcano plot of Pks13 proximity hits with mycolic acid enzymes highlighted

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Michael Glickman @michaelglickman.bsky.social · 25/03/2025

Allison reasoned that placing the ALFA tag on the target protein and expressing the high affinity anti-ALFA nanobody fused to various functional domains in live mycobacteria would be a versatile system. We show that such an approach enables faitful localization of proteins, here shown for MmpL3...

localization of Mmpl3 by direct fusion to GFP or by GFP-NBALFA localization to MmpL3-ALFA

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Michael Glickman @michaelglickman.bsky.social · 06/02/2024

Agree, very fun. I set the word frequency to 3. 🦠 #microsky

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Michael Glickman @michaelglickman.bsky.social · 09/12/2023

Important point, the name is misleading, as most names are. Here is the cdc map from 2019 for spotted fever group rickettsiosis.

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