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mfeltes.bsky.social

@mfeltes.bsky.social
19 followers 16 following 7 posts
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mfeltes.bsky.social @mfeltes.bsky.social · 15/07/2025
Check it out in #PLOSGenetics: journals.plos.org/plosgenetics...
journals.plos.org
Phenotype to genotype: A new and rapid approach using whole-genome sequencing
Author summary Forward genetic mutagenesis screening is an unbiased approach for the identification of mutations linked to a phenotype of interest. While this approach can be a powerful tool for uncov...
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mfeltes.bsky.social @mfeltes.bsky.social · 15/07/2025
We've used WheresWalker to map our newly discovered #darkyolk zebrafish mutants which have defects in #ApoB-lipoprotein metabolism. We map zion to slc3a2a, a heterodimeric amino acid transporter, and find that several SLC3a2/SLC7s are associated with ApoB phenotypes in humans.
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mfeltes.bsky.social @mfeltes.bsky.social · 15/07/2025
Validated in #zebrafish & #maize, this pipeline uses a combination of #mapping-by-sequencing and #recombinantmapping to narrow the number of candidate genes which can be tested using #F0crispr
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mfeltes.bsky.social @mfeltes.bsky.social · 15/07/2025
Announcing WheresWalker! A fast, accurate mutation-mapping pipeline that accelerates gene discovery with forward genetic screening.
journals.plos.org
Phenotype to genotype: A new and rapid approach using whole-genome sequencing
Author summary Forward genetic mutagenesis screening is an unbiased approach for the identification of mutations linked to a phenotype of interest. While this approach can be a powerful tool for uncov...
120
mfeltes.bsky.social @mfeltes.bsky.social · 28/04/2025
Read more about the screen that led to our mutant discoveries, and the mapping tool (#WheresWalker) we're using to map our loci here: www.biorxiv.org/content/10.1...
biorxiv.org
WheresWalker: a pipeline for rapid mutation mapping using whole genome sequencing
Forward genetic screening is a powerful approach to assign functions to genes and can be used to elucidate the many genes whose functions remain unknown. Chemical mutagenesis is an unbiased and effici...
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mfeltes.bsky.social @mfeltes.bsky.social · 28/04/2025
Using forward genetic screening, we discovered 28 novel zebrafish mutants @zebrafishrock.bsky.social and named them after our parks @altnps.bsky.social 🏞️🏞️. Our adventure into this biological wilderness is just beginning - we can't wait to share what we'll discover.
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mfeltes.bsky.social @mfeltes.bsky.social · 28/04/2025
Last week was #NationalParkWeek! To celebrate - meet our National Park inspired #zebrafish mutants. From acadia to zion, our “dark yolk” mutants are shedding light 💡on new biology that will help us to better understand how diabetes and cardiovascular disease develop.
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