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Jakob Meier-Credo

@meiercredo.bsky.social

95 followers 117 following 10 posts

Mass spec enthusiast | Frankfurt | Main Focus on Imaging MS, Proteomics, Lipidomics, Metabolomics

Posts Replies Media

Reposted by Jakob Meier-Credo

Katharina Geißler @kgeissler.bsky.social · 21/04/2026

Out now in Nature Communications! We show that Dictyostelium vaults associate with ER and NE membranes in situ and encapsulate ribosomes with defined orientations, shining new light on their cellular function! #TeamTomo #TeamMassSpec #ProtistsOnSky www.nature.com/articles/s41...

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Reposted by Jakob Meier-Credo

Katharina Geißler @kgeissler.bsky.social · 16/12/2025

It’s out! Using cryo-ET in Dicty cells, we take a fresh in situ look at vaults. Surprisingly, we uncover vaults associated with ER and NE membranes, and find that many vaults enclose ribosomes in defined orientations, opening new avenues to their cellular function! www.biorxiv.org/lookup/conte...

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Jakob Meier-Credo @meiercredo.bsky.social · 19/09/2025

Check out our new preprint on an integrated pipeline combining in situ #cryo-ET with MALDI #MSImaging for single-cell identification and classification from previously analysed EM-grids. Link: www.biorxiv.org/content/10.1...

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Jakob Meier-Credo @meiercredo.bsky.social · 23/07/2025

Only used it for small molecule and imaging datasets so far: mzmine mzio.io It reads timstof files natively and is super fast compared to data analysis.

mzio.io

mzio – Accelerate Scientific Discoveries

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Jakob Meier-Credo @meiercredo.bsky.social · 03/04/2025

As permanent lock-mass or injectable?

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Jakob Meier-Credo @meiercredo.bsky.social · 01/04/2025

That's quite a difference! - Wanted to test that for our Tims files, but the automatic file conversion in RawBeans produces insanely large mzml files that crash later on. Is there a specific setting / how do you convert the raw files for that?

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Jakob Meier-Credo @meiercredo.bsky.social · 27/03/2025

All of this was a preview on ASMS releases this year, but no idea when it'll practically be available...

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Jakob Meier-Credo @meiercredo.bsky.social · 27/03/2025

Bruker showed a lot on their DE/EU user meeting already: -Entry level metabolomics TIMS; with low mass capable quad -Biopharma (Omnitrap-)TIMS -AIP ion processor upgrade (improved ion transfer CC->TOF) -More robust nanoLC (nanoResolve?), an upgraded nE2 with ceramic valves, AS pump & inline filters

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Jakob Meier-Credo @meiercredo.bsky.social · 19/03/2025

It elutes rather late in the gradient / wash step - not sure if it would come off an Evotip. For our workflow it also gets filtered out as a +1 charge in ion mobility.

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Jakob Meier-Credo @meiercredo.bsky.social · 15/01/2025

An easy way to test is either by trap injection (higher equilibration volume for flushing out air) or to set the air gaps to "0" in the advanced settings

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Jakob Meier-Credo @meiercredo.bsky.social · 15/01/2025

Does it only occur during runs or also with idle flow at these solvent compositions? Did you recently update the LC-Plugin? We've had similar issues after that (2.1 SR2) and found a software bug, where to much air is drawn during the injection, which creates spray instabilities.

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Jakob Meier-Credo @meiercredo.bsky.social · 09/02/2024

www.shimadzu.com/an/products/...

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