The dissociation of the streptavidin-biotin (SA-b) bond has been widely characterized using bulk and single molecule force spectroscopy (SMFS) techniques. However, the dissociation rates ( k off) from...

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The kinetics of assembly and Ca2+-dependent disassembly of Syt-1 oligomers, determined by quantitative single molecule analysis, meet or exceed the physiologic requirements to function as a clamp.