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SapKrap

@justanonymous.bsky.social
73 followers 184 following 41 posts

CryoEM postdoc | Kansas | TRP channel fan | www.sebk.space

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SapKrap @justanonymous.bsky.social · 29/09/2026
Out of curiosity I looked up the researchgate of the friend I mentioned, and they have 19 authorships (not including posters/platforms). Looking at the peers and recent grads of the same medical school (that I could find online) and the range is between 1 to 20s. I guess it's context dependent.
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SapKrap @justanonymous.bsky.social · 29/09/2026
Could be! But I asked this person several times (in disbelief) and she was insistent that 30 was the number. I've also asked MD/PhD students at my institution and they've said 30 is crazy but the expectation now is for more pubs especially for competitive residencies.
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SapKrap @justanonymous.bsky.social · 29/09/2026
Depending on the field it could be a career defining amount! I don't know if it's the case at all institutions or all specialties, however.
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SapKrap @justanonymous.bsky.social · 28/09/2026
A family friend is in med school and wants to do derm. She told me the expectation for competitive applicants is 30 journal articles, so at least ten a year. I don't know any other field where this would be considered normal or acceptable.
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SapKrap @justanonymous.bsky.social · 22/09/2026
From what I've heard he was already rude, abrasive and absent when he represented the big 1st. Pat Roberts barely scraped by after his residency scandal so I'm not sure what Roger was thinking. If the polling with Cindy H is to be believed Roger really is a legendarily bad candidate
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SapKrap @justanonymous.bsky.social · 21/05/2026
You can't really understand your structure and what it means unless YOU actually model it and think about it. Thinking back years ago, an elder xtalographer told me that phenix autobuild ruined a generation of structural biologists. Don't think there is a direct equivalency to this moment though.
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SapKrap @justanonymous.bsky.social · 31/03/2026
Great to see a CNTN paper, excellent work!
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SapKrap @justanonymous.bsky.social · 28/03/2026
He's just trying to get a slice of the Rod Dreher electorate
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SapKrap @justanonymous.bsky.social · 27/01/2026
Allscripts? I'm not sure. This is very complex software that has no forgiveness for failure in a very regulated environment. Brent Shafer basically destroyed Cerner through contempt for human capital and this was compounded by the people from Oracle. I don't think old Cerner is recoverable.
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SapKrap @justanonymous.bsky.social · 11/01/2026
I'm reliant on 1.2/1.3 300 mesh; any deviations I can't really work around. I know SubAngstrom was making 1.8/2 300 mesh some time back, I wonder if they're making other sizes now. Also, Single Particle LLC offered good pricing for ANTcryos last I asked, maybe it would be worth getting some...
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SapKrap @justanonymous.bsky.social · 11/01/2026
Same experience! I did get some trial packs from Mitegen but they were fragile and required optimization beyond my patience. Lots of torn squares but I did get some nice particles.
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SapKrap @justanonymous.bsky.social · 19/10/2025
I had something growing in a SDS stock solution years back and I just put it in the back of a drawer. If you want to grow in a 10% surfactant solution you deserve respect.
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SapKrap @justanonymous.bsky.social · 11/10/2025
She would be 79 if elected. I don't know what victory Chuck thinks he has here. Not since the 1920s or Reconstruction has the Democratic party had such sclerotic and weak leadership. www.axios.com/2025/10/09/u...
axios.com
Scoop: Janet Mills to launch Senate bid Tuesday, per internal document
Mills' entrance into the race is also likely to set off a divisive primary contest.
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SapKrap @justanonymous.bsky.social · 25/04/2025
www.nature.com/articles/d41... Never knew you could use worms for expression!
nature.com
A protein tunnel that shuttles lipids around the cell
The high-resolution structure of a ‘superhighway’ for lipids in cells has been obtained.
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SapKrap @justanonymous.bsky.social · 17/04/2025
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SapKrap @justanonymous.bsky.social · 26/03/2025
Absolutely appalling, so sorry to hear this Jason.
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SapKrap @justanonymous.bsky.social · 22/03/2025
My 4 y/o told me something no parent ever wants to hear. "I want to be a protein scientist with a microscope like you". He's too young to understand you could be a C elegans geneticist or an architect and not someone that spends weeks freezing aggregates on rare metal disks.
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SapKrap @justanonymous.bsky.social · 13/02/2025
What a stupid country www.washingtonpost.com/health/2025/...
washingtonpost.com
RFK Jr. confirmed, elevating anti-vaccine activist to nation’s top health post
The Senate held a final vote on whether to approve Robert F. Kennedy Jr. for Health and Human Services secretary after a three-month confirmation fight.
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SapKrap @justanonymous.bsky.social · 07/02/2025
This is really such a hopeless political party. www.rollingstone.com/politics/pol...
rollingstone.com
Dem Overseeing 'Digital Assets' to Hold Luxury Retreat With Crypto Kingpin
Arizona Sen. Ruben Gallego, who’s overseeing a “digital assets” committee, is set to hold a luxury weekend donor retreat with a crypto kingpin.
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SapKrap @justanonymous.bsky.social · 04/02/2025
I should have been clearer in my reply, I was referring to the paper in my post. I'm not a prolific author at all, but I have had demands where it was clear that nothing was enough for the reviewers. It appears that Mignot and Cambillau didn't have such demands from Nature for more experiments.
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SapKrap @justanonymous.bsky.social · 04/02/2025
AF has been of use at a gross level for reconstructions, but for my projects the predictions have been rather poor. I don't know enough about the subject matter in the paper, but if I were to publish something similar I would only feel comfortable with complimentary experiments to prove my points.
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SapKrap @justanonymous.bsky.social · 31/01/2025
www.nature.com/articles/s42... This is the first "all AlphaFold and nothing else" paper I've seen
nature.com
Structural model of a bacterial focal adhesion complex - Communications Biology
The structural model of a bacterial focal adhesion complex reveals the connection of essential functional modules throughout the cell envelope and offer an inspiring view of the force transduction mec...
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SapKrap @justanonymous.bsky.social · 23/01/2025
Old Outlook: Click calendar entry->open attachment New Outlook: Click calendar entry->opens browser window->organization SSO->opens Office 365 calendar -> click calendar entry -> you do not have permission to open this file
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SapKrap @justanonymous.bsky.social · 07/01/2025
If you're still there, Maru Maru in Papeete was a major highlight for us
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SapKrap @justanonymous.bsky.social · 07/01/2025
I was there last year, can't wait to go back.
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SapKrap @justanonymous.bsky.social · 06/01/2025
Moorea?
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SapKrap @justanonymous.bsky.social · 07/12/2024
Congratulations Brandon!
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SapKrap @justanonymous.bsky.social · 02/10/2024
Every time JD Vance talks I feel an uncomfortable hum in my ears.
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SapKrap @justanonymous.bsky.social · 26/06/2024
Combining cryoEM and functional studies we found that mammalian TRPM5 exhibit unique properties and are uniquely sensitive to calcium. Coupling of MHR domains seems to influence gating, but we'll need more TRPM structures to determine this.
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SapKrap @justanonymous.bsky.social · 26/06/2024
Very happy to show the first work published from the Zubcevic Lab at KUMC. www.pnas.org/doi/10.1073/...
pnas.org
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SapKrap @justanonymous.bsky.social · 17/06/2024
An entire day wasted because of Unicorn 7.1! I don't know why Cytiva needs to make the most cumbersome software. I've spoken to people at dozens of institutions who like Aktas but have hated the Unicorn install.
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SapKrap @justanonymous.bsky.social · 09/06/2024
In my hometown of Springfield, MO for the first time in a long time and the fattest man I've ever seen is wearing a shirt with an AR-15 on it that says "Socialism Preventer"
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SapKrap @justanonymous.bsky.social · 19/05/2024
Never thought I would say this, but Phenix on Windows has not crashed once this project whereas Phenix on my Ubuntu box can only run from command line.
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SapKrap @justanonymous.bsky.social · 10/04/2024
www.nature.com/articles/s41... What a delightful structural paper, so happy to see it published
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SapKrap @justanonymous.bsky.social · 22/01/2024
From Andrew Fire, Viroid-like colonists of human microbiomes www.biorxiv.org/content/10.1...
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SapKrap @justanonymous.bsky.social · 01/12/2023
www.nature.com/articles/s41...
nature.com
AlphaFold predictions are valuable hypotheses and accelerate but do not replace experimental structu...
An analysis of AlphaFold protein structure predictions shows that while in many cases the predictions are highly accurate, there are also many instances where the predicted structures or parts of pred...
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SapKrap @justanonymous.bsky.social · 03/11/2023
Half a year wait for Quantifoils. Outrageous that wait times have never improved, only gotten worse. Has anyone ever used C-flats? Are they about the same or worse?
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SapKrap @justanonymous.bsky.social · 02/11/2023
Sometimes I have real apprehension about the future of experimental structural biology. The predictions for my proteins of interest are currently poor and some domains are nonsensically arranged. Who is to say this couldn't change in ten, maybe five years though?
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SapKrap @justanonymous.bsky.social · 13/10/2023
www.theatlantic.com/ideas/archiv... An exemplary piece really hitting home how only morons should read The Atlantic. Who on earth could possibly give a shit about what some depraved neoconservative from the Bush years thinks about the Middle East.
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SapKrap @justanonymous.bsky.social · 03/10/2023
After seeing a talk I realize how much I miss crystallography sometimes. There's nothing like shooting a poor looking crystal and getting a beautiful array of spots back. After two years of membrane proteins I also realize how much I miss soluble proteins that can be worked with.
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SapKrap @justanonymous.bsky.social · 25/09/2023
Writing a NIH BioSketch is such a humiliating exercise
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