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John F. Rawls @jfrawls.bsky.social · 04/08/2026

The Rawls Lab is hiring! We are seeking a motivated research technician to support cutting-edge studies in gastrointestinal physiology using zebrafish & mice. To learn more about this hands-on & in-person position at Duke University in Durham NC, click here: careers.duke.edu/job/Durham-R...

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John F. Rawls @jfrawls.bsky.social · 24/07/2026

We're excited to share this new article describing the major findings of the Pediatric Obesity Microbiome & Metabolism Study (POMMS). Also very grateful to have been able to undertake this interesting project w/ such a talented and generous interdisciplinary team! medschool.duke.edu/news/scienti...

medschool.duke.edu

Scientists are discovering something surprising about obesity in teenagers

The teen years may be a critical window when the gut microbiome and its influence on weight is still taking shape and more open to change.

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John F. Rawls @jfrawls.bsky.social · 14/03/2026

Cherry blossoms in Duke Gardens - a gentle and reliable lesson in impermanence

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John F. Rawls @jfrawls.bsky.social · 28/02/2026

Please share w/ interested colleagues this new EMBO Course in Bath UK (6-18 July 2026) “Computational & experimental microbiomics”: lnkd.in/e7EGH4RG The course includes experimental emphasis on zebrafish host-microbiome interaction + general microbiomics skills. Register is 11 May #EMBOMicrobiomics

lnkd.in

This link will take you to a page that’s not on LinkedIn

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John F. Rawls @jfrawls.bsky.social · 12/02/2026

Happy International Day of Women & Girls in Science! Today & everyday I am grateful for the opportunity to work alongside & learn from this talented group of women scientists. Funded by grants from the NIH, this team is working to understand how the gut microbiome affects our digestive health.

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Reposted by John F. Rawls

PLOS Biology @plosbiology.org · 19/12/2025

Enteroendocrine cells (EECs) in the #gut #epithelium secrete diverse hormones that regulate digestion, energy use & behavior. @jfrawls.bsky.social &co identify EEC subtypes & #hormone profiles in #zebrafish, and explore their lineage relationships @plosbiology.org 🧪 plos.io/49iZKWC

Enteroendocrine cell subtypes in zebrafish: A zebrafish larva at 6 days postfertilization expressing transgenic reporters neurod1:TagRFP, neurogenin3:QF2, and QUAS:GFP. neurod1:TagRFP labels the pancreatic islet and all enteroendocrine cells in the gut and is shown in magenta. The combination of neurogenin3:QF2 and QUAS:GFP reporters labels a subset of enteroendocrine cells in the gut and is shown in cyan. Overlapping signal appears white. Image credit: Margaret Morash.

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John F. Rawls @jfrawls.bsky.social · 18/12/2025

Two new articles from our lab!! Davis et al identified epithelial transcription factor ELF3 as a key mediator of host-microbiota interaction in zebrafish tinyurl.com/pkavnxdp Morash et al revealed enteroendocrine cell subtypes in zebrafish & catalogued their peptide hormones tinyurl.com/bdzbm2xm

tinyurl.com

Identification of intestinal enteroendocrine cell subtypes and their associated hormones in zebrafish

Enteroendocrine cells (EECs) in the gut epithelium secrete diverse hormones that regulate digestion, energy use, and behavior. This study identifies EEC subtypes and their hormone profiles in zebrafis...

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John F. Rawls @jfrawls.bsky.social · 30/10/2025

Part 7 & End of Chapter: While waiting for export permits, we explored the Kathmandu Valley—alive with Buddhist & Hindu traditions, temples at every turn, chaotic traffic, hills filled with with monasteries, & endless momos. Fieldwork now complete, the analysis phase begins. Thanks for following!

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John F. Rawls @jfrawls.bsky.social · 28/10/2025

Part 6: For decades, the zebrafish has helped scientists unlock the mysteries of genetics, development, & disease. But the fish that power most discoveries have spent generations inside lab tanks. This video captures footage of zebrafish in their natural environment in Nepal. youtu.be/cCZhZpPALqw

youtu.be

Zebrafish in the wild

YouTube video by John Rawls

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John F. Rawls @jfrawls.bsky.social · 26/10/2025

Part 5: Amid the fieldwork & meetings, this trip also brought our U.S. & Nepali teams closer together. The visit happened to overlap with the Tihar festival, giving us time to see the celebrations firsthand & to share meals & conversations that connected the work to the culture & people around it.

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John F. Rawls @jfrawls.bsky.social · 24/10/2025

Part 4: Our fieldwork focused on wild zebrafish, but in our downtime we couldn’t resist meeting some of Nepal’s other remarkable fauna. A highlight for me was seeing the critically endangered gharial crocodile up close at Chitwan’s Gharial Breeding Center.

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John F. Rawls @jfrawls.bsky.social · 23/10/2025

Part 3: Agriculture & Forestry Univ. (AFU) was an outstanding partner & hub for this study. Their faculty generously shared lab space, equipment, & expertise essential to this work. We were especially impressed by the AFU students, whose curiosity & initiative energized the project. Dhanyabaad, AFU!

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John F. Rawls @jfrawls.bsky.social · 22/10/2025

Part 2: With expert guidance from our AFU partners, we explored Chitwan’s maze of streams, canals, and wetlands. Using a range of capture methods, we found zebrafish thriving in many locations. Stay tuned for movies!

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John F. Rawls @jfrawls.bsky.social · 22/10/2025

Part 1: The last couple of weeks I've had the pleasure of working with a team of scientists from Duke University, University of Oregon, and Agriculture and Forestry University (AFU) in Nepal to study the biology of zebrafish in their natural habitat. ...

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John F. Rawls @jfrawls.bsky.social · 28/08/2025

Another RawlsLab retreat is in the books! Activities included centering our core values & skill development goals, science communication, a postapocalyptic scavenger hunt, & enjoying the NC summer. Now more than ever, I’m deeply grateful to work alongside this team of talented & intrepid scientists.

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John F. Rawls @jfrawls.bsky.social · 22/04/2025

Congratulations to Briana Davis and Maggie Morash on their successful PhD thesis defenses in the Duke University Molecular Genetics and Microbiology Program!

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John F. Rawls @jfrawls.bsky.social · 08/03/2025

Happy International Women’s Day! Today and everyday I am grateful for the opportunity to work alongside and learn from this talented group of women scientists. Funded by grants from the NIH, this team is working to understand how the gut microbiome affects our digestive and metabolic health.

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