Julia Bubis @jbubis.bsky.social · 10/11/2025Spotted at Ion Opticks booth🤣 yeah, I am at #HUPO2025. Let’s chat :) 050
Julia Bubis @jbubis.bsky.social · 20/03/2025At Uni, at Mike Gorshkov lab. First good practical skills I gained in Denmark, at SDU. Then reading articles helped a lot:) And I am still learning:) 020
Julia Bubis @jbubis.bsky.social · 21/02/2025I just wanted to share some painful experience related to weird mass accuracy, that we faced in the past, and which took us quite some time to investigate:) Maybe it will be helpful for someone else:) 010
Julia Bubis @jbubis.bsky.social · 21/02/2025However, if data with different CVs and for each CV mass acc differs, the distribution will be bimodal. So the correction will be either suboptimal or need to be done separately for each CV. 110
Julia Bubis @jbubis.bsky.social · 21/02/2025Oh, yeah:) let’s me paraphrase. My example isn’t the cause of this strange behaviour. It’s additional layer, which can cause similar strange behaviour:) for this data, one can nicely correct mass shifts. 000
Julia Bubis @jbubis.bsky.social · 20/02/2025I’m a bit surprised that somebody remembers this article:) thank you:) 020
Julia Bubis @jbubis.bsky.social · 20/02/2025It's also good to check mass acc for different Compensation Voltages separately. We also once had raw files, where mass acc was shifted only for one CV, and completely ok for the other. 120
Julia Bubis @jbubis.bsky.social · 16/01/2025I guess you can find the right answer in the author list😀 in this case, the intuition won't fail for sure😀 120
Julia Bubis @jbubis.bsky.social · 16/01/2025Finally, it is out!:) #SCP #Astral #massspectromentry www.nature.com/articles/s41...nature.comChallenging the Astral mass analyzer to quantify up to 5,300 proteins per single cell at unseen accuracy to uncover cellular heterogeneity - Nature MethodsAn ultra-low-input single-cell proteomic workflow is optimized to yield maximum proteome coverage with high accuracy and precision on the Orbitrap Astral mass spectrometer. 1195
