iiinova — where your digital self keeps evolving

Reposted by Jake Leyhr

Genetics Society of America @genetics-gsa.bsky.social · 24/06/2026

🔍️ Explore a new #proteome resource in the June issue of #G3journal 🪱 WormTagDB, an interactive resource, consolidates all known endogenously tagged alleles in #Celegans and provides #CRISPR guides and knock-ins across all protein-coding genes. @jakeleyhr.bsky.social 🔗 buff.ly/tMsF0Uk

032

Reposted by Jake Leyhr

Journal of Anatomy @journalofanatomy.bsky.social · 09/07/2025

The Weberian apparatus boosts hearing in otophysan fish via modified bones and ligaments. Using advanced imaging and histology, a study from @jakeleyhr.bsky.social and @remorphevolab.bsky.social reveals new insights into the elusive first intercostal ligament and its role in this acoustic system

144

Jake Leyhr @jakeleyhr.bsky.social · 18/01/2025

Here's some rendered anterior and ventral views of the crazy jaw and toothplates in a young juvenile 🤩 (Lower jaw in red, upper jaw in sky blue)

0254

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

We end the paper with suggestions for future comparative studies of ICL1 and the function of the OSS. We hope that 3D models like ours will lead to more functional/FEA analyses to understand better the intricacies of fish hearing! 15/15

000

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

We also note that, reflective of its anchoring role, rib/vertebra 5 appears like a “transitional vertebra” between the WA and the other thoracic vertebra, bearing in mind that in other species (e.g. catfishes), it is much more heavily modified as part of the WA. 14/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

Based on the orientation and composition of ICL1, we argue that it should be included in the category of “Weberian ligaments”, likely functioning to reduce “loose" or "wasted” motion of the OSS and increase the sound sensitivity of the WA. 13/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

To my knowledge, this is the first detailed 3D imaging of the triple ligament that tethers together the OSS, tripus, and the anterior face of the swim bladder. 12/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

We described the juvenile-adult development of ICL1 along with the other Weberian ligaments, showing they are all mature by 10mm SL. 11/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

Histologically, ICL1 closely resembles the interossicular ligament (IOL), with densely packed, taut collagen-1 fibers (unlike the elastin-rich suspensor ligament; SUS), suggesting it strains to hold rib 4/oss in place. 10/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

We found that ICL1 bridges features of Weberian and posterior intercostal ligaments, with a unique medial attachment at a steep angle aligned with the os suspensorium (OSS), unlike the slack, flattened posterior ICLs (green). 9/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

I wondered if ICL1 could be stabilising the WA but I couldn’t find any mention of this in the literature, so, we reached out to Nathan Bird, an expert on the WA and its histology, and began the super fun collaboration that led to this paper. 8/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

Realising it was an intercostal ligament (ICL1), I segmented more posterior ICLs to compare it with, but ICL1 stood out in terms of shape and orientation as it connected the WA to rib 5. 7/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

While I was segmenting the area slice by slice, working in axial/sagittal/coronal views, I noticed a small hint of soft tissue running backwards from the fourth rib, and when I segmented it, I saw that it connected rib 4 to rib 5. 6/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

The story begins with our 2023 paper using contrast-enhanced synchrotron scanning to describe soft-tissue defects in zebrafish mutants (www.frontiersin.org/journals/end...), in which I spent a lot of time segmenting the Weberian apparatus. 5/

frontiersin.org

Frontiers | Enhanced contrast synchrotron X-ray microtomography for describing skeleton-associated soft tissue defects in zebrafish mutants

Detailed histological analyses are desirable for zebrafish mutants that are models for human skeletal diseases, but traditional histological techniques are l...

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

Since being first described more than 200 years ago, the anatomy and diversity of the WA in Otophysi have been well-characterised. We review this prior work in our paper, but our focus is on describing in detail a ligament that is almost entirely absent from the literature. 4/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

The swim bladder functions as a resonating chamber, collecting and amplifying sound waves from the environment, transmitting them through the Weberian ossicles and ligaments to the fluid in the ear, leading to the movement of otoliths and stimulation of auditory hair cells. 3/

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

The Weberian apparatus (WA) refers primarily to the four heavily modified cervical vertebrae of Otophysan fish (10,000 species) that form a chain of small bones and interconnecting ligaments to connect the swim bladder to the inner ear. 2/

Weberian ligaments (shades of purple) connecting the bony ossicles to the fluid-filled chambers of the ear (sinus perilymphaticus – pink, sinus endolymphaticus – green).

100

Jake Leyhr @jakeleyhr.bsky.social · 17/01/2025

🚨 New(ish 😅) publication alert! 🚨 Out now in the latest issue of the Journal of Anatomy, we described an almost completely overlooked ligament in the Zebrafish Weberian apparatus, and its potential impact on sound transmission 🐟🔊: onlinelibrary.wiley.com/doi/10.1111/... Let’s dive in! 🧵 1/

onlinelibrary.wiley.com

The overlooked first intercostal ligament: Does it help to stabilize the Weberian apparatus?

3D rending of adult zebrafish Weberian apparatus in association with the occiput, swim bladder, and rib-bearing vertebrae, with highlighted interossicular, suspensor, and intercostal ligaments.

131

Reposted by Jake Leyhr

BIOM @biom-banyuls.bsky.social · 16/12/2024

🎉 New publication from the DEV team at BIOM! The sensory shark: High-quality data on Scyliorhinus canicula reveal the evolution of sensory organs in jawed vertebrates, published in Molecular Biology and Evolution (2024). 🦈✨ 👉 doi.org/10.1093/molb... Congrats ! #catshark #BIOM #evolution #genome

doi.org

The sensory shark: high-quality morphological, genomic and transcriptomic data for the small-spotted catshark Scyliorhinus canicula reveal the molecular bases of sensory organ evolution in jawed verte...

Abstract. Cartilaginous fishes (chondrichthyans: chimaeras and elasmobranchs -sharks, skates and rays) hold a key phylogenetic position to explore the orig

165