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Luke Pattison @interlukein.bsky.social · 23/06/2026

Really excited to share out latest preprint! We’ve been studying how the proton-sensing GPCR GPR65 drives inflammatory pain – and found that *where* it signals matters! Huge thanks to support from @mayadannawi.bsky.social and @psalmotoxin.bsky.social! Read here: www.biorxiv.org/content/10.6...

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Luke Pattison @interlukein.bsky.social · 02/04/2026

Still time to submit your latest work on #inflammatorypain to our special issue of Cells focussed on the Molecular Mechanisms of Inflammatory Pain. We’re accepting manuscripts until September - if you have something suitable in the pipeline, please get in touch!

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Reposted by Luke Pattison

Ewan St. John Smith @psalmotoxin.bsky.social · 19/11/2025

At 13:00 our @phar.cam.ac.uk lab will be live @sfn.org! Poster N3 sees @mayadannawi.bsky.social presenting on overexpression of Prdm12 in the adult sensory nervous system Poster O6 sees @interlukein.bsky.social presenting on loss of Piezo2 in colonic sensory neurons with age Please drop on by 😃

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Luke Pattison @interlukein.bsky.social · 15/11/2025

Excited for @sfn.org #SfN2025 in not-so-sunny-today San Diego! 🧠 👨‍🔬 🔬 I’ll be presenting our latest work on Piezo2 👈, colonic control 💩 and aging 👵🏻 on Wednesday, 1-5 pm at board O6 - come say hi! 🙃

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Luke Pattison @interlukein.bsky.social · 02/10/2025

Great summary of our latest work - a real Team Science effort! 👥 🐭

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Luke Pattison @interlukein.bsky.social · 26/09/2025

I am currently curating a Special Issue for MDPI Cells on “Molecular Mechanisms Underlying Inflammatory Pain.” If you are working on, or planning a suitable manuscript, I would be delighted if you would consider submitting it to our issue. mdpi.com/journal/cell...

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Luke Pattison @interlukein.bsky.social · 25/09/2025

A new academic year! 🍏 Proud to watch my Pegasus Scholars students presenting on targeted cancer therapies today, after a week exploring the formation, functions, faults and fixing of proteins! 🔬 Glad to support Robinson College’s excellent widening participation initiation for another year! 👨‍🏫

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Luke Pattison @interlukein.bsky.social · 05/05/2025

If you have #osteoarthritis, please see below 👇 - I’m running a 2 minute survey and would love your input 😇

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Luke Pattison @interlukein.bsky.social · 22/04/2025

👋I’m developing a proposal to investigate how pain changes with progression of osteoarthritis (OA). 🦴⚡️ If you have OA, I’d love to hear how your experience with pain has changed over time - please consider answering this quick 10-question survey! 🙏 forms.office.com/e/pYwbTrD3Qv

forms.office.com

Microsoft Forms

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Luke Pattison @interlukein.bsky.social · 10/02/2025

🤩 geeking out at Geneva light festival last weekend - double helix 🧬 & neuron 🧠 #genevalux

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Reposted by Luke Pattison

Luke Pattison @interlukein.bsky.social · 02/01/2025

📣 Calling fellow ECRs studying the contributions of GPCRs to pain and/or analgesia! 🧠 Interested in presenting at #SfN25 in San Diego in November? 🙏 Please drop me a message - looking for last few speakers for a mini symposium proposal! #PainResearch #neuroskyance

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Luke Pattison @interlukein.bsky.social · 02/01/2025

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Luke Pattison @interlukein.bsky.social · 11/12/2024

I’m so happy to see this out! ☺️ I’ve been working on this >6 years - many setbacks along the way, but they've made getting this published all the more rewarding - perseverance is worth it! 💪 Very thankful for the support of @psalmotoxin.bsky.social, @mayadannawi.bsky.social & others! 🧵[10/10]

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Luke Pattison @interlukein.bsky.social · 11/12/2024

Taken together, we postulate that GPR65 on FLS orchestrates inflammatory joint pain through the release of mediators and subsequent crosstalk between neurons and immune cells. Thus inhibiting GPR65 might represent a means of relieving inflammatory pain. 🧵[9/10]

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Luke Pattison @interlukein.bsky.social · 11/12/2024

Increased expression of GPR65 has previously been reported in human osteoarthritis (OA). BTB also stimulated increases in pro-inflammatory cytokine production by FLS isolated from human OA patients. Further, synovial fluid samples from OA patients could activate GPR65. 🧵[8/10]

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Luke Pattison @interlukein.bsky.social · 11/12/2024

The role of GPR65 was confirmed by the inability of BTB to increase inflammatory mediator secretion by GPR65 knockout (KO) FLS, and lack of inflammation and pain-like behaviours when GPR65 KO mice were injected with BTB. 🧵[7/10]

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Luke Pattison @interlukein.bsky.social · 11/12/2024

…suggesting another cell type might be involved. Fibroblast-like synoviocytes (FLS), which line the knee joint, express GPR65. Stimulating FLS with BTB increased secretion of inflammatory cytokines, which could increase the excitability of naïve neurons. 🧵[6/10]

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Luke Pattison @interlukein.bsky.social · 11/12/2024

To investigate the cellular basis of the pain-like behaviours seen in mice, we explored whether BTB increased neuronal excitability. While neurons isolated from BTB-injected mice were hyperexcitable, we could not recreate this by incubating naïve neurons with BTB alone… 🧵[5/10]

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Luke Pattison @interlukein.bsky.social · 11/12/2024

Next, we injected BTB to the knee joint of mice. This caused both inflammation and an increase in pain-like behaviours, suggesting that activation of GPR65 in the knee joint is sufficient to drive inflammatory pain. 🧵[4/10]

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Luke Pattison @interlukein.bsky.social · 11/12/2024

…So, we first compared the signalling signatures of protons, BTB and psychosine (all reported to activate GPR65). We found that BTB recapitulated most features of proton-mediated signalling. Importantly, BTB was also shown to only activate GPR65 over other PS-GPCRs. 🧵[3/10]

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Luke Pattison @interlukein.bsky.social · 11/12/2024

Inflammatory conditions, like arthritis, are both painful and associated with acidosis. Thus we investigated if acidosis drives pain through GPR65 (a proton-sensing GPCR) - this isn’t an easy task though, as there are lots of receptors that can be activated by protons… 🧵 [2/10]

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Luke Pattison @interlukein.bsky.social · 11/12/2024

Riding the year out on a high ✨ - latest paper out now in @pnas.org! ☺️ 🙏 Here we describe a role for the proton-sensing GPCR, GPR65 in mediating joint pain from fibroblast-like synoviocytes! www.pnas.org/doi/10.1073/... #PainResearch #Neuroskyence More on this work below 👇 🧵 [1/10]

pnas.org

Activation of the proton-sensing GPCR, GPR65 on fibroblast-like synoviocytes contributes to inflammatory joint pain | PNAS

Inflammation is associated with localized acidosis, however, attributing physiological and pathological roles to proton-sensitive receptors is chal...

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Luke Pattison @interlukein.bsky.social · 21/11/2024

Excellent walk-through of our latest work, looking at lipids and inflammatory joint pain 👇

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Luke Pattison @interlukein.bsky.social · 21/11/2024

👋 Hello, I'm new here... ...followed the herd to a greener field! 🐏 🦋 I'm a postdoc interested in the mechanisms driving inflammatory pain, with the aim of identifying new points of intervention, for the development of better medicines! #PainResearch #FirstPost

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