My penultimate PhD project is out in @natchembio.nature.com! We engineered LaccID, a cell surface-specific enzyme for proximity labeling and EM. Derived from an ancestral laccase, it performs the same chemistry as APEX2 but using O2 instead of H2O2.
www.nature.com/articles/s41...
nature.com
Directed evolution of LaccID for cell surface proximity labeling and electron microscopy - Nature Chemical Biology
LaccID, an engineered laccase, enables hydrogen-peroxide-free proximity labeling and electron microscopy (EM) in mammalian cells. Notably, LaccID is selectively active at the cell surface, enabling th...