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Reposted by Functional Metabolomics Lab

Daniel Petras @daniel-petras.bsky.social · 07/02/2026

I am super excited that our paper on inter-laboratory comparability of non-targeted LC–MS/MS analysis of dissolved organic matter was just published in ES&T. pubs.acs.org/doi/10.1021/...

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 27/11/2025

A huge thank you to Nike and the Forchhammer Lab and to Khaled Selim and all co-authors, especially our Natural-Product-Partner-in-Crime Chambers Hughes for solving the structure of 2’-deoxyxanthopterin B2, and Karoline Steuer-Lodd and the Ansel Hsiao lab for generating the mutants.

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 27/11/2025

Our data show that CutA binds copper more strongly in the presence of pteridines, suggesting that these metabolites act as co-factors that modulate copper binding and redox homeostasis.

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 27/11/2025

New paper from our team: Led by @nike-wagner.bsky.social, we just published our findings on how the metabolite class of pteridines binds to the previously cryptic CutA protein. We used our native metabolomics approach to identify pteridines as ligands of CutA. www.pnas.org/doi/10.1073/...

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 26/10/2025

Combining the new tool with the bioluminescent bioreporter strains from Heike Brötz-Oesterhelt and her students Julian Schubert and Leonard Boldt was a key element to achieve the high sensitivity of the bioactivity assays needed for microfractions.

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 26/10/2025

Most importantly, the paper wouldn't have been possible without Christian Geibel, who led the project, optimized the method, and pushed the paper over the finish line. Thanks a lot Christian and everybody else at the University of Tuebingen and UC Riverside who were involved in the project 🧡🧡🧡

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 26/10/2025

Another key component was the analysis software, which Simon Knoblauch, back then a master’s student in our group, built and which, thanks to the help of @mingxunwang.bsky.social, is now available through @gnps2.bsky.social.

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 26/10/2025

Its great to see the project in completion after almost four years ago, when Albert Hernandez, a mechanical engineer from UCSD, joined us in Tübingen, and started building the microspotter.

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 26/10/2025

This was a large interdisciplinary effort together with the groups of Heike Brötz-Oesterhelt within the @cmfi.bsky.social Tübingen, and @mingxunwang.bsky.social at @ucriverside.bsky.social.

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 26/10/2025

Making use of high-speed, low-volume fractionation, we can achieve similar sampling rates for bioactivity measurements as for the mass spec DDA cycles, which enables correlation of LC-MS and LC-bioactivity data and pinpoints individual active metabolites. www.youtube.com/watch?v=PTC9...

youtube.com

Microspotter1.0

YouTube video by Functional Metabolomics Lab

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 26/10/2025

We are super excited that our new paper on microfluidic-based LC-MS/MS fractionation in combination with bioluminescence bioreporters readouts, for compound-resolved bioactivity metabolomics, was just published: pubs.acs.org/doi/10.1021/...

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Reposted by Functional Metabolomics Lab

Daniel Petras @daniel-petras.bsky.social · 27/07/2025

New paper from the group. Together with Chambers Hughes, Giovanni Vitale and our amazing collaborators, we developed a multiplexed chemical metabolomics workflow to assign functional groups in non-targeted LC-MS/MS data: www.nature.com/articles/s41... Behind the paper story: go.nature.com/45ljV4d

nature.com

Enhancing tandem mass spectrometry-based metabolite annotation with online chemical labeling - Nature Communications

To improve annotation in non-targeted metabolomics studies, authors develop a Multiplexed Chemical Metabolomics (MCheM) platform, combining post-column derivatization with integrated data processing. ...

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 04/07/2025

We are super excited to start a new project with support from the Simons Foundation. We will leverage our metabolomics tools and SynCom models to study how microbes shape the production, transformation, and fate of marine DOM. Thanks so much @simonsfoundation.org‬ for making this possible!

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 23/05/2025

We are pretty stoked that our paper on chemical shifts in kelp forests in the Gulf of Maine made it onto the front cover of ‪@science.org Big congratulations to Shane Farrell and everybody involved! ✌️✌️✌️

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Reposted by Functional Metabolomics Lab

ICTC13 @ictc13.bsky.social · 07/05/2025

Exciting invited talk by Daniel Petras @daniel-petras.bsky.social Functional #Metabolomics Approaches for Understanding Microbial Community Dynamics at the Molecular Level. #ICTC13 @func-metabo-lab.bsky.social

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Reposted by Functional Metabolomics Lab

ICTC13 @ictc13.bsky.social · 13/04/2025

Another success story of native MS. Pteridines, found also in #cyanobacteria, bind to cutA, possibly bridging copper homeostasis and cellular redox regulation. doi.org/10.1101/2025... More about functional metabolomics at #ICTC13 from @daniel-petras.bsky.social (Invited speaker).

doi.org

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 04/04/2025

This was a big collaborative effort with multiple groups from @cmfi.bsky.social (Forchhammer, Selim, Link, Hughes) and @maxplanck.de Tuebingen (Hartmann), from microbiology phenotyping to metabolomics, natural product structure elucidation, and biophysical interaction studies. 🧡

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 04/04/2025

We screened cell extracts from the cyanobacterium Synechococcus, for interaction with the homotrimer CutA, which can be found across the tree of life. Our results identified pteridines, to be ligands which enhances CutA copper binding and redox balance.

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 04/04/2025

New reprint from the team: Lead by @nike-wagner.bsky.social, we used our native metabolomics setup to shed new light onto the function of the CutA protein. www.biorxiv.org/content/10.1...

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 02/03/2025

There will be also a remote participation option and all sessions will be recorded. The recordings from our past summer schools are on youtube and linked on our web page: www.functional-metabolomics.com/resources

functional-metabolomics.com

Resources

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 02/03/2025

Good news: We will have another summer school on non-targeted metabolomics this year! Organized by Martin Hansen and Scott Jarmusch, this year's summer school will be hosted at DTU in Copenhagen from August 18-22, inc. a summer camp style accommodation option at DTU. Sign up: tinyurl.com/NTMS2025

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 12/02/2025

New preprint from our collaboration with @aliciaperezlorente.bsky.social from Diego Romero's group, who spend some time with us at @cmfi.bsky.social. It's great to see all the puzzle pieces coming together, providing a detailed snapshot of the molecular interplay between Bacillus and and Botrytis.

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 17/12/2024

We are very proud that after a long repair/upgrade and moves across continents, our most critical piece of equipment is fully functional again. 💪💪💪 Big thanks to Jorge and Karo for fixing it and for generating the tutorial. ❤️ www.youtube.com/watch?v=Bf43...

youtube.com

La Pavoni - Espresso Tutorial

YouTube video by Functional Metabolomics Lab

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 25/11/2024

In case you missed part 2 of our our #mzmine seminar series on @ansgarkorf.bsky.social on: - LC-MS² - GC/EI-MS - MALDI imaging the recording is online here: www.youtube.com/watch?v=cGA5... Coming up next: Dec 3rd, 5 PM CET / 8 AM PST @steffenheu.bsky.social on analyzing LC-MS and LC-IMS-MS data

youtube.com

VMOL Seminar #29 - mzmine series: 2. Multimodal Data Processing (Ansgar Korf)

YouTube video by Functional Metabolomics Lab

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 14/11/2024

Next Tuesday, Nov 19, 5 pm CET / 8 am PST we will have the next #VMOL seminar #mzmine series on multimodal data processing using mzwizard. And in case you wana watch the last mzmine seminar on non-targeted LC-MS/MS data processing, you can find the recording here: youtu.be/UnqVtZngzl0

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 16/03/2024

We had a blast at the #ecometabolomics workshop @UniCologne last week. Thanks so much for organizing this awesome event @C_Sanchez_Arcos and for the great contribution and inspiring interactions with all participants and instructors. This was a lot of fun!

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 15/02/2024

New paper from the team, reviewing current functional metabolomics tools for natural product research. Congrats to @Giovann97116204 and Christian for spearheading the effort, and big thanks to @mingxunwang.bsky.social and @AllegraAron for doing this together. pubs.rsc.org/en/content/a...

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 25/01/2024

In case you wana learn more about our non-targeted LC-MS/MS pipeline in the context of Eco-Metabolomics, sign up and stop by the upcoming workshop organized by @C_Sanchez_Arcos at the @UniCologne sites.google.com/view/eco-met...

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Functional Metabolomics Lab @func-metabo-lab.bsky.social · 30/12/2023

New preprint by @diegoromerohi, @davidmicron, @APerezLorente, et al., showcasing the effect of bacterial cyclodipeptides on fungal membrane stability. Congratulations to everyone involved, and thank you for having us be a part of the team. ✌️✌️ www.biorxiv.org/content/10.1...

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