Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/20256/ These findings establish TGIF2 as a major regulator of NSC fate, integrating transcriptional repression with lineage priming to fine-tune neurogenesis. Looking forward to discussions and feedback! 🔬💡 010
Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/20255/ Importantly, TGIF2 is not just a repressor of differentiation—it plays an active role in neurogenic priming. By keeping neurogenic priming genes at low levels to keep NSCs poised but undifferentiated, ensuring proper lineage priming. 110
Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/20254/ Overexpressing TGIF2 increased NSCs in vitro and in vivo, delaying differentiation. scRNA-seq, Cut&Run, and interactome analyses revealed that TGIF2 recruits the SIN3A/HDAC repressor complex to suppress key neuronal differentiation and maturation genes. 110
Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/20253/ TGIF2 stood out—it was the only TF whose expression and motif enrichment correlated with “neurogenesis”, independent of brain region and time. 110
Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/20252/ We profiled NSCs from the mouse embryonic cortex and lateral ganglionic eminence (LGE) at the peak of neurogenesis and at birth (when cortex transitions to gliogenesis but LGE continues neurogenesis) using RNA-seq and ATAC-seq to identify key regulators. 110
Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/20251/ NSCs express neurogenic genes at low levels, but how do they prevent premature differentiation while staying poised for neurogenesis? How is the balance between self-renewal and differentiation regulated? 110
Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/2025🚀 Excited to share our latest work uncovering TGIF2 as a key transcription factor (TF) safeguarding neural stem cell (NSC) fate and regulating neurogenic priming! 🧠 🔗 biorxiv.org/content/10.1101/2025.02.13.635953v1 Here’s how we found it and why it’s important!🧵👇biorxiv.orgTGIF2 is a major regulator of neural stem cell fate and neurogenic primingDuring brain development, neural stem cells (NSCs) must balance self-renewal with differentiation and ensure lineage progression. To identify novel regulators of NSCs during neurogenesis, we isolated ... 2104
Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/20254/ Overexpressing TGIF2 increased NSCs in vitro and in vivo, delaying differentiation. scRNA-seq, Cut&Run, and interactome analyses revealed that TGIF2 recruits the SIN3A/HDAC repressor complex to suppress key neuronal differentiation and maturation genes. 000
Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/20253/ TGIF2 stood out—it was the only TF whose expression and motif enrichment correlated with “neurogenesis”, independent of brain region and time. 100
Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/20252/ We profiled NSCs from the mouse embryonic cortex and lateral ganglionic eminence (where neurogenesis continues) at the peak and the transition to gliogenesis using RNA-seq and ATAC-seq to identify key regulators. 100
Yiling Li 李怡灵 @fionalyl.bsky.social · 17/02/20251/ NSCs express neurogenic genes at low levels, but how do they prevent premature differentiation while staying poised for neurogenesis? How is the balance between self-renewal and differentiation regulated? 100
