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Evan Healy

@evhealy.bsky.social
542 followers 377 following 14 posts

Research fellow studying all things chromatin, transcription & RNA Monash University, Melbourne, Australia 🇮🇪

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Evan Healy @evhealy.bsky.social · 14/01/2025
4/ We propose that the non-enzymatic functions of EZH1-PRC2 and canonical PRC1 complexes play a critical role in maintaining long-term repression of Polycomb target genes in non-dividing cells.
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Evan Healy @evhealy.bsky.social · 14/01/2025
3/ Our data shows that in quiescent cells (non-dividing), there’s a shift to less enzymatically active PRC1 (the canonical CBX containing forms) and PRC2 (containing EZH1). These changes appear to be evolutionarily conserved. 🧬
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Evan Healy @evhealy.bsky.social · 14/01/2025
2/ We discovered that non-dividing cells are refractory to PRC2 inhibitors, meaning H3K27me2/3 are not reduced and gene expression is unchanged. This indicates that replicative dilution, not active H3K27 demethylation, is the main mechanism for H3K27me2/3 reduction upon inhibition of PRC2🔬
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