How do intracellular parasites remodel host cells? We developed spaCR to connect parasite genes with spatial phenotypes by pooled imaging-based CRISPR screening, and used it to identify EAF1 as a Toxoplasma gondii effector involved in host ESCRT subversion.
www.biorxiv.org/content/10.6...
biorxiv.org
A pooled image-based CRISPR screen identifies EAF1 as a T. gondii modulator of ESCRT subversion.
Intracellular pathogens remodel host cells by redirecting cellular machinery to the host-pathogen interface. The protozoan parasite Toxoplasma gondii co-opts host ESCRT proteins at the parasitophorous...
