iiinova — where your digital self keeps evolving

Diego A. Márquez @dmarquez.bsky.social · 09/10/2026

🧬 I’m pleased to be presenting our work with @mabi-behruznia.bsky.social at ICCMg 2026 in Geneva this November. 🎤 “Towards hospital-ready certification of metagenomic pathogen calls” Using clinical metagenomics, biophysics and quantitative inference to validate calls under sparse genomic recovery.

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Diego A. Márquez @dmarquez.bsky.social · 21/08/2026

🌿 Unsaturation 2026 in South Lake Tahoe: a small workshop on when and how leaf air spaces become unsaturated. Thanks @tombuckleylab.bsky.social for the invitation, with support from @ucdavisplants.bsky.social and @li-corenv.bsky.social I shared our gas-exchange work on non-stomatal control. 💧

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Diego A. Márquez @dmarquez.bsky.social · 05/08/2025

Flash talks at the New Phytologist Next Generation Scientists 2025 event at the University of Birmingham, UK! Sharing insights on mesophyll conductance—how shifts in liquid-phase diffusivity shape gas exchange. @newphyt.bsky.social @unibirmingham.bsky.social

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Diego A. Márquez @dmarquez.bsky.social · 13/07/2025

Preprint with @lucascernusak.bsky.social, @florianabusch.bsky.social and Graham Farquhar! We show that mesophyll cell water status is the key signal regulating non-stomatal control of transpiration. We introduce a mechanistic model linking plant hydraulics and gas exchange. doi.org/10.1101/2025...

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Diego A. Márquez @dmarquez.bsky.social · 09/05/2025

Very excited to present our research on “Biochemical Modulation of Mesophyll Resistance” at the Gordon Research Seminar in June. Many thanks to the organisers for the opportunity. Looking forward to discussing mesophyll conductance and photosynthesis with everyone at the meeting in Newry!

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Diego A. Márquez @dmarquez.bsky.social · 14/01/2025

Excited to share our paper with Anna Gardner and @florianabusch.bsky.social in Plant Ecophysiology!🌿 We review challenges in interpreting plant stress responses using gas exchange data, offer solutions to improve accuracy and discuss gaps in our current knowledge. www.sciltp.com/journals/Pla...

$Detailed representation of H2O gas exchange (left) and CO2 gas exchange (right) at the adaxial (upper) and abaxial (lower) leaf surfaces in an amphistomatous leaf. This panel illustrates the separate gas exchange processes for each leaf surface, which can be measured individually if the upper and lower cuvettes are analysed separately—though this setup is not common in commercial gas exchange systems. Stomatal, cuticle, and boundary layer resistances are depicted for both surfaces, along with the mole fractions of water vapour and CO2.$

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Diego A. Márquez @dmarquez.bsky.social · 06/12/2024

I just finished the amazing EMBL Hands-on Proteomics Curse! It was packed with practical sessions on sample prep, instrument demos, and bioinformatics, plus excellent theoretical insights. Huge thanks to the brilliant instructors and organisers! 🙌 #EMBLProteomics

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Diego A. Márquez @dmarquez.bsky.social · 16/11/2024

My first post in BSky, let's do it about our last paper (doi.org/10.1073/pnas...). We show how vital nonstomatal control of transpiration is for maintaining high assimilation rates in plants such as maize and sorghum under increased VPD. This mechanism boosts C4 plants' water-use efficiency.

Diagram of the mechanism of nonstomatal control of transpiration as VPD increases, generating unsaturation in the substomatal cavity. The parameters in the figure are atmospheric water vapour concentration (wa), water vapour concentration on the leaf surface (ws), substomatal cavity water vapour concentration (wi), water vapour concentration at saturation (wsat), resistance to water diffusion through the plasma membrane (rp), cell wall water potential (Ψw), and cytosolic water potential (Ψc). Black dots represent cell wall fibres. The current theory suggests that aquaporins acting as transmembrane conduits close when VPD rises, increasing the resistance to water movement through the membrane of mesophyll cells (rp) and triggering unsaturation in the substomatal cavity.

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